Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.

Plasmacytoid dendritic cells (pDCs) produce massive quantities of type-I interferon (IFN) in response to viral an infection or self nucleic acids. Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8), additionally referred to as CMRF-35-like molecule-6 (CLM-6), is a putative activating receptor amongst mouse LMIR/CLM/CD300 members; nevertheless, the expression and performance of LMIR8 stay unclear.

Here, we characterize mouse LMIR8 as a pDC receptor. Analysis of Flag-tagged LMIR8-transduced bone marrow (BM)-derived mast cells demonstrated that LMIR8 can transmit an activating sign by interacting with immunoreceptor tyrosine-based activating motif (ITAM)-containing FcRγ.

Flow cytometric evaluation utilizing a particular antibody for LMIR8 confirmed that LMIR8 expression was restricted to mouse pDCs residing in BM, spleen, or lymph node. FcRγ deficiency dampened floor expression of LMIR8 in mouse pDCs. Notably, LMIR8 was detected solely in pDCs, no matter TLR9 stimulation, suggesting that LMIR8 is an acceptable marker for pDCs in mouse tissues; LMIR8 is weakly expressed in Flt3 ligand-induced BM-derived pDCs (BMpDCs).

Annexin V-Cy3 Apoptosis Kit
K102-400	Biovision	each	1149.6 EUR
Annexin V-Cy5 Apoptosis Kit
K103-100	Biovision	each	614.4 EUR
Annexin V-Cy5 Apoptosis Kit
K103-25	Biovision	each	261.6 EUR
Annexin V-Cy5 Apoptosis Kit
K103-400	Biovision	each	1227.6 EUR
Annexin V-Cy3 Apoptosis Kit
K2004-100	ApexBio	100 assays	619.2 EUR
Annexin V-Cy3 Apoptosis Kit
K2004-25	ApexBio	25 assays	285.6 EUR
Annexin V-Cy3 Apoptosis Kit
K2004-400	ApexBio	400 assays	1336.8 EUR
Annexin V-Cy5 Apoptosis Kit
K2005-100	ApexBio	100 assays	619.2 EUR
Annexin V-Cy5 Apoptosis Kit
K2005-25	ApexBio	25 assays	285.6 EUR
Annexin V-Cy5 Apoptosis Kit
K2005-400	ApexBio	400 assays	1336.8 EUR
Annexin V-FITC Apoptosis Kit
K101-100	Biovision	each	614.4 EUR
Annexin V-FITC Apoptosis Kit
K101-25	Biovision	each	268.8 EUR
Annexin V-FITC Apoptosis Kit
K101-400	Biovision	each	1227.6 EUR
Annexin V-EGFP Apoptosis Kit
K104-100	Biovision	each	601.2 EUR
Annexin V-EGFP Apoptosis Kit
K104-25	Biovision	each	248.4 EUR
Annexin V-EGFP Apoptosis Kit
K104-400	Biovision	each	1149.6 EUR
Annexin V-FITC Apoptosis Kit
K2003-100	ApexBio	100 assays	619.2 EUR
Annexin V-FITC Apoptosis Kit
K2003-25	ApexBio	25 assays	285.6 EUR
Annexin V-FITC Apoptosis Kit
K2003-400	ApexBio	400 assays	1336.8 EUR
Annexin V-EGFP Apoptosis Kit
K2006-100	ApexBio	100 assays	619.2 EUR
Annexin V-EGFP Apoptosis Kit
K2006-25	ApexBio	25 assays	285.6 EUR
Annexin V-EGFP Apoptosis Kit
K2006-400	ApexBio	400 assays	1336.8 EUR
Annexin V-Biotin Apoptosis Kit
K109-100	Biovision	each	561.6 EUR
Annexin V-Biotin Apoptosis Kit
K109-25	Biovision	each	248.4 EUR
Annexin V-Biotin Apoptosis Kit
K109-400	Biovision	each	1149.6 EUR
Annexin V-Biotin Apoptosis Kit
K2009-100	ApexBio	100 assays	619.2 EUR
Annexin V-Biotin Apoptosis Kit
K2009-25	ApexBio	25 assays	285.6 EUR
Annexin V-Biotin Apoptosis Kit
K2009-400	ApexBio	400 assays	1336.8 EUR
Annexin V Apoptosis Detection Kit (PE)
55R-1288	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (PE)
abx290003-100test	Abbexa	100 test	693.6 EUR
Annexin V Apoptosis Detection Kit (PE)
abx290015-200test	Abbexa	200 test	644.4 EUR
Annexin V Apoptosis Detection Kit (CY3)
55R-1266	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (CY5)
55R-1267	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (FITC)
55R-1265	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (FITC)
abx290002-100test	Abbexa	100 test	644.4 EUR
Annexin V Apoptosis Detection Kit (FITC)
abx290014-200test	Abbexa	200 test	577.2 EUR
Annexin V-PE Apoptosis Kit Plus
K203-100	Biovision	each	582 EUR
Annexin V-PE Apoptosis Kit Plus
K203-25	Biovision	each	280.8 EUR
Annexin V-PE Apoptosis Kit Plus
K203-400	Biovision	each	1136.4 EUR
Annexin V-PE Apoptosis Kit Plus
K2059-100	ApexBio	100 assays	619.2 EUR
Annexin V-PE Apoptosis Kit Plus
K2059-25	ApexBio	25 assays	301.2 EUR
Annexin V-PE Apoptosis Kit Plus
K2059-400	ApexBio	400 assays	1270.8 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K202-100	Biovision	each	588 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K202-25	Biovision	each	288 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K202-400	Biovision	each	1136.4 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K2058-100	ApexBio	100 assays	619.2 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K2058-25	ApexBio	25 assays	301.2 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K2058-400	ApexBio	400 assays	1270.8 EUR
Annexin V Apoptosis Detection Kit (biotin)
55R-1271	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (Biotin)
abx290017-200test	Abbexa	200 test	744 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-100	Biovision	each	601.2 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-25	Biovision	each	288 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-400	Biovision	each	1136.4 EUR
Annexin V-FITC Apoptosis Kit Plus
K2057-100	ApexBio	100 assays	619.2 EUR
Annexin V-FITC Apoptosis Kit Plus
K2057-25	ApexBio	25 assays	301.2 EUR
Annexin V-FITC Apoptosis Kit Plus
K2057-400	ApexBio	400 assays	1270.8 EUR
Annexin V Apoptosis Detection Kit (PE) (CY5)
55R-1289	Fitzgerald	25 assays	416.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V-PE Apoptosis Detection Kit
K128-100	Biovision	each	601.2 EUR
Annexin V-PE Apoptosis Detection Kit
K128-25	Biovision	each	248.4 EUR
Annexin V-PE Apoptosis Detection Kit
K128-400	Biovision	each	1149.6 EUR
Annexin V-PE Apoptosis Detection Kit
K2200-100	ApexBio	100 assays	619.2 EUR
Annexin V-PE Apoptosis Detection Kit
K2200-25	ApexBio	25 assays	285.6 EUR
Annexin V-PE Apoptosis Detection Kit
K2200-400	ApexBio	400 assays	1336.8 EUR
Annexin V Apoptosis Detection Kit Plus (PE)
55R-1304	Fitzgerald	25 assays	363.6 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V / PI Apoptosis Detection Kit (FITC)
abx290007-100test	Abbexa	100 test	543.6 EUR
Annexin V EGFP Apoptosis Detection Kit
55R-1268	Fitzgerald	25 assays	332.4 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit Plus (CY3)
55R-1303	Fitzgerald	25 assays	355.2 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit Plus (FITC)
55R-1302	Fitzgerald	25 assays	355.2 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V / PI Apoptosis Detection Kit (Biotin)
abx290011-100test	Abbexa	100 test	744 EUR
Annexin V Apoptosis Detection Kit (CF-Blue)
abx290018-200test	Abbexa	200 test	744 EUR
Annexin V Apoptosis Detection Kit (Dylight 634)
abx290016-200test	Abbexa	200 test	644.4 EUR
Aposcreen Annexin V Apoptosis Kit : PE (OKSB00008)
OKSB00008	Aviva Systems Biology	100 Tests	615.6 EUR
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: The ApoScreen Annexin V Apoptosis Kit employs a fluorescein- labeled annexin V (annexin V- FITC) in concert withpropidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis,cells begin to display PS on the outer cell membrane where it is readily detectable by staining the cells with annexin VFITC.As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readilymove across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lostmembrane integrity through mechanisms including necrosis. When cells are double stained with annexin V- FITC and PI,three different cell populations may be observed (Figure 2): (i) live cells that do not stain with either annexin V- FITC or PI;(ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with annexin V- FITC only. Analysis canbe performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells canbe examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.;Sensitivity:
Annexin V Apoptosis Detection Kit : PE (OKTA00005)
OKTA00005	Aviva Systems Biology	KIT	364.8 EUR
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:
Annexin V Apoptosis Detection Kit : APC (OKTA00002)
OKTA00002	Aviva Systems Biology	KIT	396 EUR
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007)
OKSB00007	Aviva Systems Biology	100 Tests	615.6 EUR
Description: Description of target: ;Species reactivity: ;Application: FC, ICC;Assay info: ;Sensitivity:
Annexin V Apoptosis Detection Kit : FITC (OKTA00003)
OKTA00003	Aviva Systems Biology	KIT	380.4 EUR
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Annexin V (FITC) / PI Apoptosis Detection Kit
abx090601-100tests	Abbexa	100 tests	552 EUR
Annexin V / 7AAD Apoptosis Detection Kit (PE)
abx290009-100test	Abbexa	100 test	594 EUR
Annexin V / PI Apoptosis Detection Kit (CF-Blue)
abx290012-100test	Abbexa	100 test	678 EUR
Annexin V-FITC/PI Apoptosis Detection Kit
A211-01	Vazyme	50 rxn	259.2 EUR
Annexin V-FITC/PI Apoptosis Detection Kit
A211-02	Vazyme	100 rxn	354 EUR
Annexin V / PI Apoptosis Detection Kit (Dylight 634)
abx290010-100test	Abbexa	100 test	644.4 EUR
Annexin V / 7ADD Apoptosis Detection Kit (FITC)
abx290008-100test	Abbexa	100 test	543.6 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-100	Biovision	each	588 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-25	Biovision	each	301.2 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-400	Biovision	each	1246.8 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-100	ApexBio	100 assays	668.4 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-25	ApexBio	25 assays	351.6 EUR
Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-400	ApexBio	400 assays	1503.6 EUR
Annexin V (APC) / PI Apoptosis Detection Kit (APC)
abx090605-100tests	Abbexa	100 tests	777.6 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-01	Vazyme	50 rxn	286.8 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-02	Vazyme	100 rxn	369.6 EUR
Annexin V (FITC) / 7-AAD Apoptosis Detection Kit
abx090603-100tests	Abbexa	100 tests	594 EUR
Annexin V-FITC / PI Cell Apoptosis Detection Kit
20-abx098246	Abbexa	292.80 EUR 376.80 EUR	25 rxns 50 rxns
Annexin V-EGFP / PI Cell Apoptosis Detection Kit
20-abx098247	Abbexa	292.80 EUR 376.80 EUR	25 rxns 50 rxns
Annexin V / 7AAD Apoptosis Detection Kit (CF-Blue)
abx290013-100test	Abbexa	100 test	678 EUR
Annexin V (PE) / 7-AAD Apoptosis Detection Kit (PE)
abx090609-100tests	Abbexa	100 tests	718.8 EUR
Annexin V (APC) / 7-AAD Apoptosis Detection Kit (APC)
abx090607-100tests	Abbexa	100 tests	811.2 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828	AAT Bioquest	100 Tests	222 EUR
Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF594 Annexin V (50 assays)
30067	Biotium	1KIT	514.8 EUR
Description: Minimum order quantity: 1 unit of 1KIT
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
22829	AAT Bioquest	100 Tests	222 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm*
22830	AAT Bioquest	100 Tests	222 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22826	AAT Bioquest	100 Tests	222 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
22824	AAT Bioquest	100 Tests	222 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
22825	AAT Bioquest	100 Tests	222 EUR
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
22827	AAT Bioquest	100 Tests	222 EUR
Annexin V (PE)
abx090613-100tests	Abbexa	100 tests	644.4 EUR
Annexin V PE
EXB0027	ExBio	100 tests	244.8 EUR
Annexin V (APC)
abx090615-100tests	Abbexa	100 tests	718.8 EUR
Annexin V APC
EXB0028	ExBio	100 tests	244.8 EUR
Annexin V (FITC)
abx090611-100tests	Abbexa	100 tests	510 EUR
Annexin V FITC
EXB0024	ExBio	100 tests	244.8 EUR
Annexin V, CF770
29046	Biotium	25ug	410.4 EUR
Description: Minimum order quantity: 1 unit of 25ug
Annexin V, CF790
29047	Biotium	25ug	439.2 EUR
Description: Minimum order quantity: 1 unit of 25ug
Annexin V siRNA
20-abx900343	Abbexa	661.20 EUR 878.40 EUR	15 nmol 30 nmol
Annexin V siRNA
20-abx907673	Abbexa	661.20 EUR 878.40 EUR	15 nmol 30 nmol
Annexin V siRNA
20-abx907674	Abbexa	661.20 EUR 878.40 EUR	15 nmol 30 nmol
Annexin V antibody
70R-11770	Fitzgerald	100 ug	632.4 EUR
Description: Rabbit polyclonal Annexin V antibody
Annexin V antibody
70R-13691	Fitzgerald	100 ug	386.4 EUR
Description: Affinity purified Rabbit polyclonal Annexin V antibody
Annexin V Antibody
49376-100ul	SAB	100ul	399.6 EUR
Annexin V Antibody
49376-50ul	SAB	50ul	286.8 EUR
Annexin V antibody
10R-7939	Fitzgerald	100 ug	516 EUR
Description: Mouse monoclonal Annexin V antibody
Annexin V antibody
20R-1522	Fitzgerald	100 ug	781.2 EUR
Description: Rabbit polyclonal Annexin V antibody
Annexin V Antibody
3357-100	Biovision	each	464.4 EUR
Annexin V Antibody
3357-30T	Biovision	each	175.2 EUR
Annexin V Antibody
DF6580	Affbiotech	200ul	420 EUR
Annexin V Antibody
F49706-0.08ML	NSJ Bioreagents	0.08 ml	140.25 EUR
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
Annexin V Antibody
F49706-0.4ML	NSJ Bioreagents	0.4 ml	322.15 EUR
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
Annexin V Antibody
F49707-0.08ML	NSJ Bioreagents	0.08 ml	140.25 EUR
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]
Annexin V Antibody
F49707-0.4ML	NSJ Bioreagents	0.4 ml	322.15 EUR
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]
Annexin V antibody
PAab09988	Lifescience Market	100 ug	463.2 EUR
Annexin V Antibody
RQ5261	NSJ Bioreagents	100ul	356.15 EUR
Description: The protein encoded by the ANXA5 gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. [RefSeq]

Crosslinking of transduced LMIR8 in BMpDCs with anti-LMIR8 antibody didn’t induce IFN-α manufacturing, however somewhat suppressed TLR9-mediated manufacturing of IFN-α. Taken collectively, these observations point out that LMIR8 is an FcRγ-coupled receptor selectively expressed in mouse tissue pDCs, which could suppress pDC activation by the popularity of its ligands.

Generation and utility of a novel InsP(3)R(1) mono-antibody from mouse.

Inositol 1, 4, 5-Trisphosphate Receptor (InsP3R) is an intracellular Ca(2+) launch channel, which broadly participates in mobile processes.

Three isoforms of InsP3R had been recognized as InsP3R1, InsP3R2, and InsP3R3. They share 60-0% protein sequence homology and kind a channel in a fashion of homotetramer or heterotetramer. Several InsP3R isoform-specific rabbit antibodies have been generated to tell apart their localization and features.

However, there is no report of such a sound antibody raised from different species. In his article, we put together a mouse monoclonal antibody in opposition to an artificial peptide with rat InsP3R1-specific carboxyl terminus sequence.

This monoclonal antibody of InsP3R1 (R1-mAb) was purified and characterised as IgG2b, which may acknowledge InsP3R1 by Western-blot (WB) evaluation and immunoprecipitate (IP) InsP3R1 from moue mind lysate examined. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody may give the constant outcomes in SH-SY5Y cells, human sperm, and mouse mind paraffin part. In abstract, we generate a mouse InsP3R1-specific IgG 2b antibody figuring out InsP3R1 in WB, IF, IHC, and IP evaluation, which gives a potential selection for detection of InsP3R1, particularly in utility of co-localization evaluation with different InsP3R isoforms or different proteins.