Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.

Plasmacytoid dendritic cells (pDCs) produce massive quantities of type-I interferon (IFN) in response to viral an infection or self nucleic acids. Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8), additionally referred to as CMRF-35-like molecule-6 (CLM-6), is a putative activating receptor amongst mouse LMIR/CLM/CD300 members; nevertheless, the expression and performance of LMIR8 stay unclear.

Here, we characterize mouse LMIR8 as a pDC receptor. Analysis of Flag-tagged LMIR8-transduced bone marrow (BM)-derived mast cells demonstrated that LMIR8 can transmit an activating sign by interacting with immunoreceptor tyrosine-based activating motif (ITAM)-containing FcRγ.

Flow cytometric evaluation utilizing a particular antibody for LMIR8 confirmed that LMIR8 expression was restricted to mouse pDCs residing in BM, spleen, or lymph node. FcRγ deficiency dampened floor expression of LMIR8 in mouse pDCs. Notably, LMIR8 was detected solely in pDCs, no matter TLR9 stimulation, suggesting that LMIR8 is an acceptable marker for pDCs in mouse tissues; LMIR8 is weakly expressed in Flt3 ligand-induced BM-derived pDCs (BMpDCs).

Annexin V-Cy3 Apoptosis Kit
K2004-25	ApexBio	25 assays	143 EUR
Description: Detects Apoptosis within 10 min.
Annexin V-Cy3 Apoptosis Kit
K2004-400	ApexBio	400 assays	810 EUR
Description: Detects Apoptosis within 10 min.
Annexin V-Cy3 Apoptosis Kit
K2004-50	ApexBio	50assays	120 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Cy5 Apoptosis Kit
K2005-100	ApexBio	100 assays	192 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Cy5 Apoptosis Kit
K2005-20	ApexBio	20assays	60 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Cy5 Apoptosis Kit
K2005-25	ApexBio	25 assays	143 EUR
Description: Detects Apoptosis within 10 min.
Annexin V-Cy5 Apoptosis Kit
K2005-400	ApexBio	400 assays	810 EUR
Description: Detects Apoptosis within 10 min.
Annexin V-Cy5 Apoptosis Kit
K2005-50	ApexBio	50assays	120 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Cy3 Apoptosis Kit
K102-100	Biovision	each	601.2 EUR
Annexin V-Cy3 Apoptosis Kit
K102-25	Biovision	each	248.4 EUR
Annexin V-Cy3 Apoptosis Kit
K102-400	Biovision	each	1149.6 EUR
Annexin V-Cy5 Apoptosis Kit
K103-100	Biovision	each	614.4 EUR
Annexin V-Cy5 Apoptosis Kit
K103-25	Biovision	each	261.6 EUR
Annexin V-Cy5 Apoptosis Kit
K103-400	Biovision	each	1227.6 EUR
Annexin V-FITC Apoptosis Kit
K2003-25	ApexBio	25 assays	143 EUR
Description: Detects Apoptosis & Necrosis within 10 min.
Annexin V-FITC Apoptosis Kit
K2003-400	ApexBio	400 assays	810 EUR
Description: Detects Apoptosis & Necrosis within 10 min.
Annexin V-EGFP Apoptosis Kit
K2006-100	ApexBio	100 assays	337.6 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-EGFP Apoptosis Kit
K2006-25	ApexBio	25 assays	136 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-EGFP Apoptosis Kit
K2006-400	ApexBio	400 assays	771.2 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-EGFP Apoptosis Kit
K104-100	Biovision	each	601.2 EUR
Annexin V-EGFP Apoptosis Kit
K104-25	Biovision	each	248.4 EUR
Annexin V-EGFP Apoptosis Kit
K104-400	Biovision	each	1149.6 EUR
Annexin V-FITC Apoptosis Kit
K101-100	Biovision	each	614.4 EUR
Annexin V-FITC Apoptosis Kit
K101-25	Biovision	each	268.8 EUR
Annexin V-FITC Apoptosis Kit
K101-400	Biovision	each	1227.6 EUR
Annexin V-EGFP Apoptosis Kit
MBS7608273-INQUIRE	MyBiosource	INQUIRE	Ask for price
Annexin V-FITC Apoptosis Kit
MBS7608274-INQUIRE	MyBiosource	INQUIRE	Ask for price
Annexin V-Biotin Apoptosis Kit
K2009-100	ApexBio	100 assays	354.4 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Biotin Apoptosis Kit
K2009-25	ApexBio	25 assays	143.2 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Biotin Apoptosis Kit
K2009-400	ApexBio	400 assays	809.6 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Biotin Apoptosis Kit
K109-100	Biovision	each	561.6 EUR
Annexin V-Biotin Apoptosis Kit
K109-25	Biovision	each	248.4 EUR
Annexin V-Biotin Apoptosis Kit
K109-400	Biovision	each	1149.6 EUR
Annexin V Apoptosis Detection Kit (PE)
55R-1288	Fitzgerald	25 assays	553 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (PE)
abx290003-100test	Abbexa	100 test	693.6 EUR
Annexin V Apoptosis Detection Kit (PE)
abx290015-200test	Abbexa	200 test	644.4 EUR
Annexin V PE Apoptosis Detection Kit
MBS697179-1Kit	MyBiosource	1Kit	320 EUR
Annexin V PE Apoptosis Detection Kit
MBS697179-5x1Kit	MyBiosource	5x1Kit	1395 EUR
Annexin V Apoptosis Detection Kit (CY3)
55R-1266	Fitzgerald	25 assays	547 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (CY5)
55R-1267	Fitzgerald	25 assays	567 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Aposcreen Annexin V Apoptosis Kit-PE
MBS679231-100Tests	MyBiosource	100Tests	405 EUR
Aposcreen Annexin V Apoptosis Kit-PE
MBS679231-5x100Tests	MyBiosource	5x100Tests	1840 EUR
Annexin V Apoptosis Detection Kit (FITC)
55R-1265	Fitzgerald	25 assays	567 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (FITC)
abx290002-100test	Abbexa	100 test	644.4 EUR
Annexin V Apoptosis Detection Kit (FITC)
abx290014-200test	Abbexa	200 test	577.2 EUR
Annexin V-APC/PI Apoptosis Kit
E-CK-A217-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/PI Apoptosis Kit
E-CK-A217-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/PI Apoptosis Kit
E-CK-A217-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/PI Apoptosis Kit
E-CK-A217-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Aposcreen Annexin V Apoptosis Kit-FITC
MBS678004-100Tests	MyBiosource	100Tests	405 EUR
Aposcreen Annexin V Apoptosis Kit-FITC
MBS678004-5x100Tests	MyBiosource	5x100Tests	1840 EUR
Annexin V-FITC/PI Apoptosis Kit
E-CK-A211-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/PI Apoptosis Kit
E-CK-A211-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/PI Apoptosis Kit
E-CK-A211-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/PI Apoptosis Kit
E-CK-A211-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/PI Apoptosis Kit
E-CK-A219-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/PI Apoptosis Kit
E-CK-A219-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/PI Apoptosis Kit
E-CK-A219-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/PI Apoptosis Kit
E-CK-A219-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/PI Apoptosis Kit
K2003-100	ApexBio	100 assays	176 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-FITC/PI Apoptosis Kit
K2003-20	ApexBio	20assays	56 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-FITC/PI Apoptosis Kit
K2003-50	ApexBio	50assays	112 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-PE Apoptosis Kit Plus
K203-100	Biovision	each	582 EUR
Annexin V-PE Apoptosis Kit Plus
K203-25	Biovision	each	280.8 EUR
Annexin V-PE Apoptosis Kit Plus
K203-400	Biovision	each	1136.4 EUR
Annexin V-PE Apoptosis Kit Plus
K2059-100	ApexBio	100 assays	619.2 EUR
Annexin V-PE Apoptosis Kit Plus
K2059-25	ApexBio	25 assays	275.2 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-PE Apoptosis Kit Plus
K2059-400	ApexBio	400 assays	1270.8 EUR
Apo Annexin V-EGFP Apoptosis Kit
MBS8506092-100Assays	MyBiosource	100Assays	335 EUR
Apo Annexin V-EGFP Apoptosis Kit
MBS8506092-25Assays	MyBiosource	25Assays	200 EUR
Apo Annexin V-EGFP Apoptosis Kit
MBS8506092-50Assays	MyBiosource	50Assays	260 EUR
Apo Annexin V-EGFP Apoptosis Kit
MBS8506092-5x100Assays	MyBiosource	5x100Assays	1530 EUR
Annexin V Apoptosis Detection Kit (biotin)
55R-1271	Fitzgerald	25 assays	513 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V Apoptosis Detection Kit (Biotin)
abx290017-200test	Abbexa	200 test	744 EUR
Annexin V-FITC/PI Apoptosis Detection Kit Annexin V-FITC/PI
40302ES08	Yeasen Biotechnology	5T	65 EUR
Annexin V-FITC/PI Apoptosis Detection Kit Annexin V-FITC/PI
40302ES20	Yeasen Biotechnology	20 T	232 EUR
Annexin V-FITC/PI Apoptosis Detection Kit Annexin V-FITC/PI
40302ES50	Yeasen Biotechnology	50 T	422 EUR
Annexin V-FITC/PI Apoptosis Detection Kit Annexin V-FITC/PI
40302ES60	Yeasen Biotechnology	100 T	602 EUR
Annexin V-EGFP/PI Apoptosis Detection Kit Annexin V-EGFP/PI
40303ES20	Yeasen Biotechnology	20 T	232 EUR
Annexin V-EGFP/PI Apoptosis Detection Kit Annexin V-EGFP/PI
40303ES50	Yeasen Biotechnology	50 T	422 EUR
Annexin V-EGFP/PI Apoptosis Detection Kit Annexin V-EGFP/PI
40303ES60	Yeasen Biotechnology	100 T	602 EUR
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-Cy3 Apoptosis Kit Plus
K202-100	Biovision	each	588 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K202-25	Biovision	each	288 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K202-400	Biovision	each	1136.4 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K2058-100	ApexBio	100 assays	915.2 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-Cy3 Apoptosis Kit Plus
K2058-25	ApexBio	25 assays	301.2 EUR
Annexin V-Cy3 Apoptosis Kit Plus
K2058-400	ApexBio	400 assays	1270.8 EUR
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC Apoptosis Kit Plus
K2057-100	ApexBio	100 assays	619.2 EUR
Annexin V-FITC Apoptosis Kit Plus
K2057-25	ApexBio	25 assays	313.6 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-FITC Apoptosis Kit Plus
K2057-400	ApexBio	400 assays	1270.8 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-100	Biovision	each	601.2 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-25	Biovision	each	288 EUR
Annexin V-FITC Apoptosis Kit Plus
K201-400	Biovision	each	1136.4 EUR
Annexin V -AF555/ SYTOX apoptosis kit
EGQ0033	EnoGene	50T	225 EUR
Annexin V-Cyanine5/PI Apoptosis Kit
E-CK-A221-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/PI Apoptosis Kit
E-CK-A221-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/PI Apoptosis Kit
E-CK-A221-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/PI Apoptosis Kit
E-CK-A221-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-AF555/SYTOX apoptosis kit
MBS8584931-50Tests	MyBiosource	50Tests	305 EUR
Annexin V-AF555/SYTOX apoptosis kit
MBS8584931-5x50Tests	MyBiosource	5x50Tests	1480 EUR
Annexin V Apoptosis Detection Kit (PE) (CY5)
55R-1289	Fitzgerald	25 assays	540 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V-PE Apoptosis Detection Kit
K2200-100	ApexBio	100 assays	614.4 EUR
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits
Annexin V-PE Apoptosis Detection Kit
K2200-25	ApexBio	25 assays	285.6 EUR
Annexin V-PE Apoptosis Detection Kit
K2200-400	ApexBio	400 assays	1336.8 EUR
Annexin V-PE Apoptosis Detection Kit
K128-100	Biovision	each	601.2 EUR
Annexin V-PE Apoptosis Detection Kit
K128-25	Biovision	each	248.4 EUR
Annexin V-PE Apoptosis Detection Kit
K128-400	Biovision	each	1149.6 EUR
Annexin V APC Apoptosis Detection Kit
MBS697180-1Kit	MyBiosource	1Kit	345 EUR
Annexin V APC Apoptosis Detection Kit
MBS697180-5x1Kit	MyBiosource	5x1Kit	1510 EUR
Annexin V Apoptosis Detection Kit Plus (PE)
55R-1304	Fitzgerald	25 assays	533 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V / PI Apoptosis Detection Kit (FITC)
abx290007-100test	Abbexa	100 test	543.6 EUR
Annexin V EGFP Apoptosis Detection Kit
55R-1268	Fitzgerald	25 assays	513 EUR
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V

Crosslinking of transduced LMIR8 in BMpDCs with anti-LMIR8 antibody didn’t induce IFN-α manufacturing, however somewhat suppressed TLR9-mediated manufacturing of IFN-α. Taken collectively, these observations point out that LMIR8 is an FcRγ-coupled receptor selectively expressed in mouse tissue pDCs, which could suppress pDC activation by the popularity of its ligands.

Generation and utility of a novel InsP(3)R(1) mono-antibody from mouse.

Inositol 1, 4, 5-Trisphosphate Receptor (InsP3R) is an intracellular Ca(2+) launch channel, which broadly participates in mobile processes.

Three isoforms of InsP3R had been recognized as InsP3R1, InsP3R2, and InsP3R3. They share 60-0% protein sequence homology and kind a channel in a fashion of homotetramer or heterotetramer. Several InsP3R isoform-specific rabbit antibodies have been generated to tell apart their localization and features.

However, there is no report of such a sound antibody raised from different species. In his article, we put together a mouse monoclonal antibody in opposition to an artificial peptide with rat InsP3R1-specific carboxyl terminus sequence.

This monoclonal antibody of InsP3R1 (R1-mAb) was purified and characterised as IgG2b, which may acknowledge InsP3R1 by Western-blot (WB) evaluation and immunoprecipitate (IP) InsP3R1 from moue mind lysate examined. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody may give the constant outcomes in SH-SY5Y cells, human sperm, and mouse mind paraffin part. In abstract, we generate a mouse InsP3R1-specific IgG 2b antibody figuring out InsP3R1 in WB, IF, IHC, and IP evaluation, which gives a potential selection for detection of InsP3R1, particularly in utility of co-localization evaluation with different InsP3R isoforms or different proteins.