Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.

Plasmacytoid dendritic cells (pDCs) produce massive quantities of type-I interferon (IFN) in response to viral an infection or self nucleic acids. Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8), additionally referred to as CMRF-35-like molecule-6 (CLM-6), is a putative activating receptor amongst mouse LMIR/CLM/CD300 members; nevertheless, the expression and performance of LMIR8 stay unclear.

Here, we characterize mouse LMIR8 as a pDC receptor. Analysis of Flag-tagged LMIR8-transduced bone marrow (BM)-derived mast cells demonstrated that LMIR8 can transmit an activating sign by interacting with immunoreceptor tyrosine-based activating motif (ITAM)-containing FcRγ.

Flow cytometric evaluation utilizing a particular antibody for LMIR8 confirmed that LMIR8 expression was restricted to mouse pDCs residing in BM, spleen, or lymph node. FcRγ deficiency dampened floor expression of LMIR8 in mouse pDCs. Notably, LMIR8 was detected solely in pDCs, no matter TLR9 stimulation, suggesting that LMIR8 is an acceptable marker for pDCs in mouse tissues; LMIR8 is weakly expressed in Flt3 ligand-induced BM-derived pDCs (BMpDCs).

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Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits

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Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits

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Annexin V-Cy3 Apoptosis Kit Plus

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Description: Detects Apoptotic, Necrotic & Healthy cells within 10 min

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Description: Detects Apoptotic, Necrotic & Healthy cells within 10 min

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Crosslinking of transduced LMIR8 in BMpDCs with anti-LMIR8 antibody didn’t induce IFN-α manufacturing, however somewhat suppressed TLR9-mediated manufacturing of IFN-α. Taken collectively, these observations point out that LMIR8 is an FcRγ-coupled receptor selectively expressed in mouse tissue pDCs, which could suppress pDC activation by the popularity of its ligands.

Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.
Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.

Generation and utility of a novel InsP(3)R(1) mono-antibody from mouse.

Inositol 1, 4, 5-Trisphosphate Receptor (InsP3R) is an intracellular Ca(2+) launch channel, which broadly participates in mobile processes.

Three isoforms of InsP3R had been recognized as InsP3R1, InsP3R2, and InsP3R3. They share 60-0% protein sequence homology and kind a channel in a fashion of homotetramer or heterotetramer. Several InsP3R isoform-specific rabbit antibodies have been generated to tell apart their localization and features.

However, there is no report of such a sound antibody raised from different species. In his article, we put together a mouse monoclonal antibody in opposition to an artificial peptide with rat InsP3R1-specific carboxyl terminus sequence.

This monoclonal antibody of InsP3R1 (R1-mAb) was purified and characterised as IgG2b, which may acknowledge InsP3R1 by Western-blot (WB) evaluation and immunoprecipitate (IP) InsP3R1 from moue mind lysate examined. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody may give the constant outcomes in SH-SY5Y cells, human sperm, and mouse mind paraffin part. In abstract, we generate a mouse InsP3R1-specific IgG 2b antibody figuring out InsP3R1 in WB, IF, IHC, and IP evaluation, which gives a potential selection for detection of InsP3R1, particularly in utility of co-localization evaluation with different InsP3R isoforms or different proteins.

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