X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins.
However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity.
Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.
Annexin V |
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E8ET1702-62 | EnoGene | 100ul | 275 EUR |
Description: Available in various conjugation types. |
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Annexin V |
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Annv | Clemente Associates LLC | 2 ML | 367.5 EUR |
Annexin V |
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ANXVCFB-200T | ImmunoStep | 200 test | 350.9 EUR |
Description: Annexin V conjugate in the selected fluorochrome |
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Annexin V |
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ANXVDY-200T | ImmunoStep | 200 test | 287.1 EUR |
Description: Annexin V conjugate in the selected fluorochrome |
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Annexin V |
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ANXVF-200T | ImmunoStep | 200 test | 259.6 EUR |
Description: Annexin V conjugate in the selected fluorochrome |
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Annexin V |
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ANXVPE-200T | ImmunoStep | 200 test | 287.1 EUR |
Description: Annexin V conjugate in the selected fluorochrome |
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Annexin V |
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abx095275-100l | Abbexa | 100 µl | 443.75 EUR |
Annexin V |
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abx095275-1ml | Abbexa | 1 ml | Ask for price |
Annexin V |
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abx095275-200l | Abbexa | 200 µl | Ask for price |
Annexin V 647 |
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E16FXP023a-100T | EnoGene | 100 tests | 433.33 EUR |
Description: Available in various conjugation types. |
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Annexin A5/Annexin V Rabbit mAb |
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A9262 | Abclonal | 200μL | 213.2 EUR |
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1). |
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Annexin A5 (Annexin V) (VAC-alpha) |
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MC-250 | Kamiya Biomedical Company | VAA-33 | Ask for price |
Annexin V (PE) |
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abx090613-100tests | Abbexa | 100 tests | 644.4 EUR |
Annexin V PE |
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EXB0027 | ExBio | 100 tests | 198 EUR |
Annexin V (APC) |
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abx090615-100tests | Abbexa | 100 tests | 718.8 EUR |
Annexin V APC |
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EXB0028 | ExBio | 100 tests | 198 EUR |
Annexin V (APC) |
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abx090615-100l | Abbexa | 100 µl | 537.5 EUR |
Annexin V (APC) |
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abx090615-1ml | Abbexa | 1 ml | Ask for price |
Annexin V (APC) |
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abx090615-200l | Abbexa | 200 µl | Ask for price |
Annexin V (FITC) |
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abx090611-100tests | Abbexa | 100 tests | 510 EUR |
Annexin V- FITC |
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E16FXP018a-100T | EnoGene | 100 tests | 400 EUR |
Description: Available in various conjugation types. |
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Annexin V FITC |
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EXB0024 | ExBio | 100 tests | 165 EUR |
Annexin V, Human |
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E34M143H | EnoGene | 5 μg | 155 EUR |
Annexin V siRNA |
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20-abx900343 | Abbexa |
|
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Annexin V siRNA |
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20-abx907673 | Abbexa |
|
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Annexin V siRNA |
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20-abx907674 | Abbexa |
|
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Annexin V, sulforhodamine 101 conjugate (Texas-Red Annexin V) |
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29002 | Biotium | 0.5ML | 332.4 EUR |
Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V antibody |
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10R-7939 | Fitzgerald | 100 ug | 444 EUR |
Description: Mouse monoclonal Annexin V antibody |
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Annexin V antibody |
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20R-1522 | Fitzgerald | 100 ug | 781.2 EUR |
Description: Rabbit polyclonal Annexin V antibody |
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Annexin V Antibody |
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3357-100 | Biovision | each | 464.4 EUR |
Annexin V Antibody |
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3357-30T | Biovision | each | 175.2 EUR |
Annexin V Antibody |
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49376 | SAB | 100ul | 499 EUR |
Annexin V Antibody |
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49376-100ul | SAB | 100ul | 399.6 EUR |
Annexin V Antibody |
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49376-50ul | SAB | 50ul | 286.8 EUR |
Annexin V Antibody |
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E2300787 | EnoGene | 100ug | 225 EUR |
Description: Available in various conjugation types. |
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Annexin V Antibody |
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DF6580 | Affbiotech | 200ul | 420 EUR |
Annexin V Antibody |
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DF6580-100ul | Affinity Biosciences | 100ul | 280 EUR |
Annexin V Antibody |
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DF6580-200ul | Affinity Biosciences | 200ul | 350 EUR |
Annexin V antibody |
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E39-09988 | EnoGene | 100ug/100ul | 225 EUR |
Description: Available in various conjugation types. |
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Annexin V Antibody |
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E300518 | EnoGene | 100ug/200ul | 275 EUR |
Description: Available in various conjugation types. |
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Annexin V antibody |
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CAF50033-100ug | Biomatik Corporation | 100ug | 312 EUR |
Annexin V antibody |
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CAF50425-100ug | Biomatik Corporation | 100ug | 338 EUR |
Annexin V antibody |
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70R-13691 | Fitzgerald | 100 ug | 519 EUR |
Description: Affinity purified Rabbit polyclonal Annexin V antibody |
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Annexin V antibody |
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70R-11770 | Fitzgerald | 100 ug | 447 EUR |
Description: Rabbit polyclonal Annexin V antibody |
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Annexin V Antibody |
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GWB-70457E | GenWay Biotech | 0.1 mg | Ask for price |
Annexin V Antibody |
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GWB-BA41FA | GenWay Biotech | 0.1 mg | Ask for price |
Annexin V Antibody |
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F49706-0.08ML | NSJ Bioreagents | 0.08 ml | 140.25 EUR |
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. |
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Annexin V Antibody |
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F49706-0.4ML | NSJ Bioreagents | 0.4 ml | 322.15 EUR |
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. |
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Annexin V Antibody |
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F49707-0.08ML | NSJ Bioreagents | 0.08 ml | 140.25 EUR |
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt] |
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Annexin V Antibody |
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F49707-0.4ML | NSJ Bioreagents | 0.4 ml | 322.15 EUR |
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt] |
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Annexin V antibody |
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PAab09988 | Lifescience Market | 100 ug | 463.2 EUR |
Annexin V Antibody |
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R30176 | NSJ Bioreagents | 100 ug | 356.15 EUR |
Description: Annexin V is also know as endonnexin II (ENX2),or placental protein 4 (PP4). It is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. Annexin V has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. It is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
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Annexin V Antibody |
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R31475 | NSJ Bioreagents | 100 ug | 356.15 EUR |
Description: Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
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Annexin V Antibody |
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RQ5261 | NSJ Bioreagents | 100ul | 356.15 EUR |
Description: The protein encoded by the ANXA5 gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. [RefSeq] |
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Annexin V, Recombinant |
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20014 | AAT Bioquest | 100 ug | 227 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V, Recombinant |
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20014-100ug | AAT Bioquest | 100 ug | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V, Recombinant |
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20015 | AAT Bioquest | 1 mg | 1540 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V, Recombinant |
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20015-1mg | AAT Bioquest | 1 mg | 1509 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V Mouse mAb |
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E2221242 | EnoGene | 100ul | 225 EUR |
Description: Available in various conjugation types. |
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Annexin V ELISA kit |
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55R-IB49646 | Fitzgerald | 96 wells | 1413.6 EUR |
Description: ELISA kit for the detection of Annexin V in the research laboratory |
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Annexin V ELISA kit |
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55R-ORG643 | Fitzgerald | 96 wells | 390 EUR |
Description: ELISA kit for the detection of Annexin V in the research laboratory |
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Annexin V Mouse mAb |
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E2222039 | EnoGene | 100ul | 255 EUR |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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Annexin V Rabbit mAb |
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59326 | SAB | 100ul | 339 EUR |
Annexin V Rabbit pAb |
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E2381706 | EnoGene | 100ul | 225 EUR |
Description: Available in various conjugation types. |
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Annexin V Rabbit pAb |
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E2382841 | EnoGene | 100ul | 225 EUR |
Description: Available in various conjugation types. |
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Annexin V Rabbit mAb |
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E2R22841 | EnoGene | 100ul | 255 EUR |
Description: Available in various conjugation types. |
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Annexin V Dyomics 647 |
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EXB0023 | ExBio | 100 tests | 220 EUR |
Annexin V-PE Reagent |
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1014-1000 | Biovision | each | 1358.4 EUR |
Annexin V-PE Reagent |
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1014-200 | Biovision | each | 483.6 EUR |
Annexin V-PE Reagent |
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E-CK-A115-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-PE Reagent |
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E-CK-A115-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-PE Reagent |
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E-CK-A115-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-PE Reagent |
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E-CK-A115-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V Solution (PE) |
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abx095273-100l | Abbexa | 100 µl | 350 EUR |
Annexin V Solution (PE) |
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abx095273-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Solution (PE) |
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abx095273-200l | Abbexa | 200 µl | Ask for price |
Annexin V-Cy3 Reagent |
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1002-1000 | Biovision | each | 1358.4 EUR |
Annexin V-Cy3 Reagent |
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1002-200 | Biovision | each | 483.6 EUR |
Annexin V-Cy5 Reagent |
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1013-1000 | Biovision | each | 1358.4 EUR |
Annexin V-Cy5 Reagent |
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1013-200 | Biovision | each | 483.6 EUR |
Annexin V, FITC Labeled |
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20030 | AAT Bioquest | 100 tests | 227 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V, FITC Labeled |
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20030-100tests | AAT Bioquest | 100 tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V-APC Reagent |
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E-CK-A117-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-APC Reagent |
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E-CK-A117-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-APC Reagent |
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E-CK-A117-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-APC Reagent |
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E-CK-A117-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Active Annexin V (ANXA5) |
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APA259Hu61 | Cloud-Clone | 10ug | 331 EUR |
Active Annexin V (ANXA5) |
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APA259Mu01 | Cloud-Clone | 10ug | 228 EUR |
Active Annexin V (ANXA5) |
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APA259Ra01 | Cloud-Clone | 10ug | 232 EUR |
Active Annexin V (ANXA5) |
|||
4-APA259Ra01 | Cloud-Clone |
|
|
Description: Recombinant Rat Annexin V expressed in: E.coli |
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Active Annexin V (ANXA5) |
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RPU55516-100ug | Biomatik Corporation | 100ug | 918.5 EUR |
Active Annexin V (ANXA5) |
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RPU55516-1mg | Biomatik Corporation | 1mg | 3619.2 EUR |
Active Annexin V (ANXA5) |
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RPU55516-50ug | Biomatik Corporation | 50ug | 556.8 EUR |
Annexin V Solution (APC) |
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abx095274-100l | Abbexa | 100 µl | 350 EUR |
Annexin V Solution (APC) |
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abx095274-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Solution (APC) |
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abx095274-200l | Abbexa | 200 µl | Ask for price |
Annexin V-EGFP Reagent |
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1004-1000 | Biovision | each | 1358.4 EUR |
Annexin V-EGFP Reagent |
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1004-200 | Biovision | each | 483.6 EUR |
Annexin V-FITC Reagent |
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1001-1000 | Biovision | each | 1495.2 EUR |
Annexin V-FITC Reagent |
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1001-200 | Biovision | each | 529.2 EUR |
Annexin V, TRITC Labeled |
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20031-100tests | AAT Bioquest | 100 tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
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Annexin V-FITC Reagent |
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E-CK-A111-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-FITC Reagent |
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E-CK-A111-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-FITC Reagent |
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E-CK-A111-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-FITC Reagent |
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E-CK-A111-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-EGFP Reagent |
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E-CK-A119-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-EGFP Reagent |
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E-CK-A119-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-EGFP Reagent |
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E-CK-A119-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-EGFP Reagent |
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E-CK-A119-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-HRP Antibody |
|||
E2700787 | EnoGene | 100ug | 225 EUR |
Description: Available in various conjugation types. |
|||
Annexin V Antibody (FITC) |
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GWB-22DB78 | GenWay Biotech | 100 TESTS | Ask for price |
anti- Annexin V antibody |
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FNab09988 | FN Test | 100µg | 658.5 EUR |
Description: Antibody raised against Annexin V |
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anti- Annexin V antibody |
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FNab00436 | FN Test | 100µg | 606.3 EUR |
Description: Antibody raised against Annexin V |
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anti- Annexin V antibody |
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FNab00437 | FN Test | 100µg | 606.3 EUR |
Description: Antibody raised against Annexin V |
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Annexin V Binding Assay |
|||
TBS2108 | Tribioscience | 100 tests | 200 EUR |
Annexin V Solution (FITC) |
|||
abx095272-100l | Abbexa | 100 µl | 300 EUR |
Annexin V Solution (FITC) |
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abx095272-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Solution (FITC) |
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abx095272-200l | Abbexa | 200 µl | 800 EUR |
Annexin V (ANXA5) Antibody |
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20-abx109257 | Abbexa |
|
|
Annexin V (ANXA5) Antibody |
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20-abx109540 | Abbexa |
|
|
Annexin V (ANXA5) Antibody |
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20-abx110998 | Abbexa |
|
|
Annexin V Binding Buffer |
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abx090621-100tests | Abbexa | 100 tests | 343.2 EUR |
Annexin V (ANXA5) Antibody |
|||
20-abx121016 | Abbexa |
|
|
Annexin V (ANXA5) Antibody |
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abx037408-100ug | Abbexa | 100 ug | 469.2 EUR |
Annexin V (ANXA5) Antibody |
|||
abx038319-100ug | Abbexa | 100 ug | 469.2 EUR |
Annexin V (ANXA5) Antibody |
|||
abx032878-400ul | Abbexa | 400 ul | 627.6 EUR |
Annexin V (ANXA5) Antibody |
|||
abx032878-80l | Abbexa | 80 µl | 343.2 EUR |
Annexin V (ANXA5) Antibody |
|||
abx032879-400ul | Abbexa | 400 ul | 627.6 EUR |
Annexin V (ANXA5) Antibody |
|||
abx032879-80l | Abbexa | 80 µl | 343.2 EUR |
Annexin V (ANXA5) Antibody |
|||
20-abx001443 | Abbexa |
|
|
Annexin V, FITC Conjugate |
|||
29001 | Biotium | 0.5ML | 274 EUR |
Description: BSA from bovine serum (cow; Bos taurus), Annexin V (recombinant produced in E. coli) |
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Annexin V, FITC Conjugate |
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29001-1 | Biotium | EA | 274 EUR |
Annexin V-Cy3 conjugate |
|||
20065-100Tests | AAT Bioquest | 100 Tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V-Cy5 conjugate |
|||
20066-100Tests | AAT Bioquest | 100 Tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V-Cy5.5 conjugate |
|||
20067-100Tests | AAT Bioquest | 100 Tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V-Cy7 conjugate |
|||
20068-100Tests | AAT Bioquest | 100 Tests | 222 EUR |
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V (ANXA5) Antibody |
|||
abx230436-100ug | Abbexa | 100 ug | 577.2 EUR |
Annexin V (ANXA5) Antibody |
|||
abx230437-100ug | Abbexa | 100 ug | 577.2 EUR |
Annexin V (ANXA5) Antibody |
|||
abx412125-01mg | Abbexa | 0.1 mg | 1078.8 EUR |
Annexin V (ANXA5) Antibody |
|||
abx414511-01mg | Abbexa | 0.1 mg | 526.8 EUR |
Annexin V-AF647 Reagent |
|||
E-CK-A113-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-AF647 Reagent |
|||
E-CK-A113-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-AF647 Reagent |
|||
E-CK-A113-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-AF647 Reagent |
|||
E-CK-A113-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-EV450 Reagent |
|||
E-CK-A133-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-EV450 Reagent |
|||
E-CK-A133-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-EV450 Reagent |
|||
E-CK-A133-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-EV450 Reagent |
|||
E-CK-A133-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-EV500 Reagent |
|||
E-CK-A135-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-EV500 Reagent |
|||
E-CK-A135-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-EV500 Reagent |
|||
E-CK-A135-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-EV500 Reagent |
|||
E-CK-A135-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-AF488 Reagent |
|||
E-CK-A137-100T | Elabscience Biotech | 100T | 190 EUR |
Annexin V-AF488 Reagent |
|||
E-CK-A137-200T | Elabscience Biotech | 200T | 298 EUR |
Annexin V-AF488 Reagent |
|||
E-CK-A137-500T | Elabscience Biotech | 500T | 660 EUR |
Annexin V-AF488 Reagent |
|||
E-CK-A137-50T | Elabscience Biotech | 50T | 110 EUR |
Annexin V-ER780 Reagent |
|||
E-CK-A139-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
Annexin V-ER780 Reagent |
|||
E-CK-A139-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-ER780 Reagent |
|||
E-CK-A139-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-ER780 Reagent |
|||
E-CK-A139-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V (ANXA5) Antibody |
|||
abx001443-100l | Abbexa | 100 µl | 475 EUR |
Annexin V (ANXA5) Antibody |
|||
abx001443-20l | Abbexa | 20 µl | 200 EUR |
Annexin V (ANXA5) Antibody |
|||
abx001443-50l | Abbexa | 50 µl | 325 EUR |
Annexin V Binding Buffer |
|||
abx090621-100l | Abbexa | 100 µl | 212.5 EUR |
Annexin V Binding Buffer |
|||
abx090621-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Binding Buffer |
|||
abx090621-200l | Abbexa | 200 µl | Ask for price |
Annexin V Solution (DY647) |
|||
abx095271-100l | Abbexa | 100 µl | 375 EUR |
Annexin V Solution (DY647) |
|||
abx095271-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Solution (DY647) |
|||
abx095271-200l | Abbexa | 200 µl | 1000 EUR |
Annexin V (ANXA5) Antibody |
|||
abx109257-100l | Abbexa | 100 µl | 162.5 EUR |
Annexin V (ANXA5) Antibody |
|||
abx109540-100l | Abbexa | 100 µl | 162.5 EUR |
Annexin V (ANXA5) Antibody |
|||
abx110998-100l | Abbexa | 100 µl | 612.5 EUR |
Annexin V (ANXA5) Antibody |
|||
abx037408-96tests | Abbexa | 96 tests | 337.5 EUR |
Annexin V (ANXA5) Antibody |
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abx038319-50mg | Abbexa | 50 mg | 337.5 EUR |
Annexin V (ANXA5) Antibody |
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abx032878-400l | Abbexa | 400 µl | 518.75 EUR |
Annexin V (ANXA5) Antibody |
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abx032879-400l | Abbexa | 400 µl | 518.75 EUR |
Annexin V (ANXA5) Antibody |
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abx148212-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Annexin V (ANXA5) Antibody |
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abx148212-596tests | Abbexa | 5 × 96 tests | Ask for price |
Pregnenolone co-treatment partially restores steroidogenesis, but does not prevent growth inhibition and increased atresia in mouse ovarian antral follicles treated with mono-hydroxy methoxychlor.
Mono-hydroxy methoxychlor (mono-OH MXC) is a metabolite of the pesticide, methoxychlor (MXC). Although MXC is known to decrease antral follicle numbers, and increase follicle death in rodents, not much is known about the ovarian effects of mono-OH MXC. Previous studies indicate that mono-OH MXC inhibits mouse antral follicle growth, increases follicle death, and inhibits steroidogenesis in vitro.
Further, previous studies indicate that CYP11A1 expression and production of progesterone (P4) may be the early targets of mono-OH MXC in the steroidogenic pathway. Thus, this study tested whether supplementing pregnenolone, the precursor of progesterone and the substrate for HSD3B, would prevent decreased steroidogenesis, inhibited follicle growth, and increased follicle atresia in mono-OH MXC-treated follicles. Mouse antral follicles were exposed to vehicle (dimethylsulfoxide), mono-OH MXC (10 μg/mL), pregnenolone (1 μg/mL), or mono-OH MXC and pregnenolone together for 96 h.
Levels of P4, androstenedione (A), testosterone (T), estrone (E1), and 17β-estradiol (E2) in media were determined, and follicles were processed for histological evaluation of atresia. Pregnenolone treatment alone stimulated production of all steroid hormones except E2.
Mono-OH MXC-treated follicles had decreased sex steroids, but when given pregnenolone, produced levels of P4, A, T, and E1 that were comparable to those in vehicle-treated follicles. Pregnenolone treatment did not prevent growth inhibition and increased atresia in mono-OH MXC-treated follicles. Collectively, these data support the idea that the most upstream effect of mono-OH MXC on steroidogenesis is by reducing the availability of pregnenolone, and that adding pregnenolone may not be sufficient to prevent inhibited follicle growth and survival.