Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of the most commonly used plasticizer, di-(2-ethylhexyl) phthalate, and is considered to be a reproductive toxicant. However, little is known about the effects of MEHP on ovarian antral follicles.
Thus, the present study tested the hypothesis that MEHP inhibits follicle growth via oxidative stress pathways. The data indicate that MEHP increases reactive oxygen species (ROS) levels and inhibits follicle growth in antral follicles, whereas N-acetylcysteine (NAC; an antioxidant) restores ROS levels to control levels and rescues follicles from MEHP-induced inhibition of follicle growth.
To further analyze the mechanism by which MEHP induces oxidative stress and inhibits follicle growth, the expression and activities of various key antioxidant enzymes (copper/zinc superoxide dismutase [SOD1], glutathione peroxidase [GPX], and catalase [CAT]) and the expression of key cell-cycle regulators (Ccnd2, Ccne1, and Cdk4) and apoptotic regulators (Bcl-2 and Bax) were compared in control and MEHP-treated follicles.
The data indicate that MEHP inhibits the expression and activities of SOD1 and GPX; does not inhibit Cat expression; inhibits the expression of Ccnd2, Ccne1, Cdk4, and Bcl-2; but increases the expression of Bax compared to controls. Furthermore, NAC blocks these toxic effects of MEHP.
Rat annexin 5, ANX-5 GENLISA ELISA
KLR0457 Krishgen 1 x 96 wells 341 EUR Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-1mg
QP8982-ye-1mg EnQuireBio 1mg 3296.4 EUR Annexin A5 (Annexin V)-Biotin
MC-252 Kamiya Biomedical Company VAA-33 Ask for price Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-10ug
QP8982-ye-10ug EnQuireBio 10ug 369.6 EUR Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-50ug
QP8982-ye-50ug EnQuireBio 50ug 500.4 EUR Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-100ug
QP8982-ye-100ug EnQuireBio 100ug 804 EUR Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-200ug
QP8982-ye-200ug EnQuireBio 200ug 1282.8 EUR Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-500ug
QP8982-ye-500ug EnQuireBio 500ug 2164.8 EUR Mouse annexin 5, ANX-5 GENLISA ELISA
KLM0502 Krishgen 1 x 96 wells 341 EUR Human Annexin 5, ANX-5 GENLISA ELISA
KBH1107 Krishgen 1 x 96 wells 286 EUR Bovine Annexin 5, ANX-5 GENLISA ELISA
KLB0304 Krishgen 1 x 96 wells 341 EUR Annexin VII (Annexin VII) Antibody
abx230439-100ug Abbexa 100 ug 610.8 EUR Annexin VIII (Annexin VIII) Antibody
abx230440-100ug Abbexa 100 ug 577.2 EUR Annexin A4/Annexin IV Rabbit mAb
A9203 Abclonal 50μL 1557.4 EUR Description: A synthetic peptide of human CD62P/P-selectin.Annexin A5/Annexin V Rabbit mAb
A9262 Abclonal 200μL 213.2 EUR Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1).Annexin VI/Annexin A6 Rabbit pAb
E2382616 EnoGene 100ul 225 EUR Description: Available in various conjugation types.Annexin A6/Annexin VI Rabbit mAb
A22038 Abclonal 500μL 83.2 EUR Description: Recombinant protein of Human PMF1.Annexin A5 (Annexin V) (VAC-alpha)
MC-250 Kamiya Biomedical Company VAA-33 Ask for price Horse ANX5(Annexin 5) ELISA Kit
EHS0047 FN Test 96T 681.12 EUR Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Horse;Sensitivity: 0.938 ng/mlRat Annexin A5(Annexin A5) ELISA Kit
NSL0938r Sunlong 96 Tests 498 EUR Goat Annexin A5(Annexin A5) ELISA Kit
NSL1380Gt Sunlong 96T 528 EUR Human Annexin A5(Annexin A5) ELISA Kit
NSL1060Hu Sunlong 96 wells 468 EUR Canine Annexin A5(Annexin A5) ELISA Kit
NSL1765Ca Sunlong 1860 wells 528 EUR Bovine Annexin A5(Annexin A5) ELISA Kit
NSL1870Bo Sunlong 96 T 528 EUR Porcine Annexin A5(Annexin A5) ELISA Kit
NSL1451Po Sunlong 96T 528 EUR Chicken Annexin A5(Annexin A5) ELISA Kit
NSL1507Ch Sunlong 1602 T 528 EUR Guinea pig Annexin A5(Annexin A5) ELISA Kit
NSL1474Gp Sunlong 96T 528 EUR Annexin V 647
E16FXP023a-100T EnoGene 100 tests 433.33 EUR Description: Available in various conjugation types.Annexin A3
E8ER1802-50 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A1
E8ER1803-70 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A7
E8ER1903-73 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin V
E8ET1702-62 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A2
E8ET1704-49 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin IV
E8ET7108-56 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A2
AP89346 SAB 1mg 2640 EUR Annexin A5
AP78464 SAB 1mg 2640 EUR Annexin A1
AP78480 SAB 1mg 2640 EUR Annexin A2
AP79238 SAB 1mg 2640 EUR Annexin A2
AP80284 SAB 1mg 2640 EUR Annexin A6
AP80377 SAB 1mg 2640 EUR Annexin A5
AP80382 SAB 1mg 2640 EUR Annexin A1
AP80399 SAB 1mg 2640 EUR Annexin A6
AP80426 SAB 1mg 2640 EUR Annexin A1
AP80428 SAB 1mg 2640 EUR Annexin A3
AP80504 SAB 1mg 2640 EUR Annexin A4
AP80514 SAB 1mg 2640 EUR Annexin A5
AP80515 SAB 1mg 2640 EUR Annexin A6
AP80516 SAB 1mg 2640 EUR Annexin A1
AP80543 SAB 1mg 2640 EUR Annexin A6
AP80574 SAB 1mg 2640 EUR Annexin A3
AP80584 SAB 1mg 2640 EUR Annexin A4
AP80585 SAB 1mg 2640 EUR Annexin A5
AP80586 SAB 1mg 2640 EUR Annexin A1
AP80590 SAB 1mg 2640 EUR Annexin A4
AP80619 SAB 1mg 2640 EUR Annexin A6
AP80630 SAB 1mg 2640 EUR Annexin A3
AP80655 SAB 1mg 2640 EUR Annexin A4
AP80657 SAB 1mg 2640 EUR Annexin A3
AP80658 SAB 1mg 2640 EUR Annexin A5
AP80659 SAB 1mg 2640 EUR Annexin A1
AP80687 SAB 1mg 2640 EUR Annexin A2
AP80694 SAB 1mg 2640 EUR Annexin A2
AP80710 SAB 1mg 2640 EUR Annexin A2
AP80745 SAB 1mg 2640 EUR Annexin A4
AP80760 SAB 1mg 2640 EUR Annexin A2
AP80800 SAB 1mg 2640 EUR Annexin A4
AP80952 SAB 1mg 2640 EUR Annexin V
Annv Clemente Associates LLC 2 ML 367.5 EUR Annexin V
ANXVCFB-200T ImmunoStep 200 test 350.9 EUR Description: Annexin V conjugate in the selected fluorochromeAnnexin V
ANXVDY-200T ImmunoStep 200 test 287.1 EUR Description: Annexin V conjugate in the selected fluorochromeAnnexin V
ANXVF-200T ImmunoStep 200 test 259.6 EUR Description: Annexin V conjugate in the selected fluorochromeAnnexin V
ANXVPE-200T ImmunoStep 200 test 287.1 EUR Description: Annexin V conjugate in the selected fluorochromeAnnexin A1
PR27207 Neuromics 2 ug 465.6 EUR Annexin A2
PR27211 Neuromics 5 ug 229.2 EUR Annexin A3
PR27212 Neuromics 5 ug 229.2 EUR Annexin A4
PR27213 Neuromics 2 ug 229.2 EUR Annexin A5
PR27214 Neuromics 5 ug 229.2 EUR Annexin A6
PR27215 Neuromics 2 ug 229.2 EUR Annexin A7
PR27216 Neuromics 5 ug 229.2 EUR Annexin A8
PR27217 Neuromics 5 ug 229.2 EUR Annexin V
abx095275-100l Abbexa 100 µl 443.75 EUR Annexin V
abx095275-1ml Abbexa 1 ml Ask for price Annexin V
abx095275-200l Abbexa 200 µl Ask for price Annexin A6
MO22207 Neuromics each 296.1 EUR Annexin A1
PR27207-B Neuromics each 337.5 EUR Annexin A2
PR27211-B Neuromics each 184.5 EUR Annexin A3
PR27212-B Neuromics each 184.5 EUR Annexin A4
PR27213-B Neuromics each 184.5 EUR Annexin A5
PR27214-B Neuromics each 184.5 EUR Annexin A6
PR27215-B Neuromics each 184.5 EUR Annexin A7
PR27216-B Neuromics each 184.5 EUR Annexin A8
PR27217-B Neuromics each 184.5 EUR Annexin A5
RA22139 Neuromics each 272.7 EUR Annexin A6
RA22143 Neuromics each 296.1 EUR Annexin Ⅰ
PA1006 Antagene 100μg 260 EUR Annexin Ⅳ
PA1007 Antagene 100μg 260 EUR Annexin Ⅴ
PA1008 Antagene 100μg 260 EUR Annexin A2
PA1348 Antagene 100μg 260 EUR Annexin Ⅰ
PA1006-S Antagene 100μg 290 EUR Description: Polyclonal Antibodies Conjugated to Sepharose BeadsAnnexin Ⅳ
PA1007-S Antagene 100μg 290 EUR Description: Polyclonal Antibodies Conjugated to Sepharose BeadsAnnexin Ⅴ
PA1008-S Antagene 100μg 290 EUR Description: Polyclonal Antibodies Conjugated to Sepharose BeadsAnnexin A2
PA1348-M Antagene 100μg 290 EUR Description: Polyclonal Antibodies Conjugated to Magnetic BeadsAnnexin A2
PA1348-S Antagene 100μg 290 EUR Description: Polyclonal Antibodies Conjugated to Sepharose BeadsAnnexin A13
E8ER1903-72 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A10
E8ET1610-57 EnoGene 100ul 275 EUR Description: Available in various conjugation types.Annexin A10
AP80493 SAB 1mg 2640 EUR Annexin A10
AP80951 SAB 1mg 2640 EUR Annexin A10
PR27208 Neuromics 5 ug 229.2 EUR Annexin A11
PR27209 Neuromics 5 ug 229.2 EUR Annexin A13
PR27210 Neuromics 2 ug 229.2 EUR Annexin A10
PR27208-B Neuromics each 184.5 EUR Annexin A11
PR27209-B Neuromics each 184.5 EUR Annexin A13
PR27210-B Neuromics each 184.5 EUR Rabbit polyclonal antibody to Annexin A1 (annexin A1)
TA308701 Origene Technologies GmbH 100 µl Ask for price Rabbit polyclonal antibody to Annexin VII (annexin A7)
TA308796 Origene Technologies GmbH 100 µl Ask for price Recombinant Human ANXA5/Annexin Ⅴ/Annexin A5 Protein
PKSH031601-100ug Elabscience Biotech 100ug 739 EUR Description: HumanRecombinant Human ANXA5/Annexin Ⅴ/Annexin A5 Protein
PKSH031601-20ug Elabscience Biotech 20ug 249 EUR Description: HumanOKRC00159-96W - Annexin A5/Annexin V ELISA Kit (Human)
OKRC00159-96W Aviva Systems Biology 96Wells 600 EUR Annexin Peptide
45-007P ProSci 0.1 mg 405.6 EUR Description: (NT) Annexin A11 PeptideAnnexin Peptide
45-008P ProSci 0.1 mg 405.6 EUR Description: (NT) Annexin A2 PeptidePolyclonal ANXA6/Annexin A6/Annexin VI Antibody (aa1-50)
APR02865G Leading Biology 0.05ml 580.8 EUR Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ANXA6/Annexin A6/Annexin VI (aa1-50). This antibody is tested and proven to work in the following applications:Annexin V (PE)
abx090613-100tests Abbexa 100 tests 644.4 EUR Annexin V PE
EXB0027 ExBio 100 tests 198 EUR Annexin-2 Antibody
39396-100ul SAB 100ul 468 EUR Annexin-2 Antibody
E300092 EnoGene 100ug/200ul 275 EUR Description: Available in various conjugation types.Annexin-2 Antibody
E312231 EnoGene 200ul 275 EUR Description: Available in various conjugation types.Annexin V (APC)
abx090615-100tests Abbexa 100 tests 718.8 EUR Annexin V APC
EXB0028 ExBio 100 tests 198 EUR Annexin V (APC)
abx090615-100l Abbexa 100 µl 537.5 EUR Annexin V (APC)
abx090615-1ml Abbexa 1 ml Ask for price Annexin V (APC)
abx090615-200l Abbexa 200 µl Ask for price Annexin V, sulforhodamine 101 conjugate (Texas-Red Annexin V)
29002 Biotium 0.5ML 332.4 EUR Description: Minimum order quantity: 1 unit of 0.5MLAnnexin V (FITC)
abx090611-100tests Abbexa 100 tests 510 EUR Annexin V- FITC
E16FXP018a-100T EnoGene 100 tests 400 EUR Description: Available in various conjugation types.Annexin V FITC
EXB0024 ExBio 100 tests 165 EUR Annexin A2/ANXA2
E21-205 EnoGene 10ug 225 EUR Description: Recombinant Human Annexin A2 is produced by our E.coli expression system and the target gene encoding Ser2-Asp339 is expressed.Annexin V, Human
E34M143H EnoGene 5 μg 155 EUR Annexin A3 siRNA
20-abx900342 Abbexa
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- 15 nmol
- 30 nmol
Annexin V siRNA
20-abx900343 Abbexa
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- 15 nmol
- 30 nmol
Annexin A3 siRNA
20-abx907669 Abbexa
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- 15 nmol
- 30 nmol
Annexin A3 siRNA
20-abx907670 Abbexa
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- 15 nmol
- 30 nmol
Annexin V siRNA
20-abx907673 Abbexa
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- 15 nmol
- 30 nmol
Annexin V siRNA
20-abx907674 Abbexa
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- Ask for price
- 15 nmol
- 30 nmol
ANXA1 (Annexin A1)
MO47023 Neuromics 100 ul 418.8 EUR Annexin A2 Human
rAP-2789 Angio Proteomie Inquiry Ask for price Recombinant human Annexin A5/Annexin V/ANXA5 Protein, His Tag
E40KMP1303 EnoGene 20ug 495 EUR Annexin A3 Protein
abx061599-200ug Abbexa 200 ug 1528.8 EUR Annexin A1 protein
30R-1291 Fitzgerald 20 ug 492 EUR Description: Purified recombinant Human Annexin A1 proteinAnnexin A5 protein
30R-1406 Fitzgerald 100 ug 267 EUR Description: Purified recombinant Human Annexin A5 proteinAnnexin A3 protein
30R-1453 Fitzgerald 100 ug 267 EUR Description: Purified recombinant Human Annexin A3 proteinAnnexin A4 protein
30R-1463 Fitzgerald 100 ug 622 EUR Description: Purified recombinant Human Annexin A4 proteinAnnexin A5 protein
30R-3209 Fitzgerald 50 ug 308.4 EUR Description: Purified recombinant Annexin A5 proteinAnnexin A1 Peptide
46-785P ProSci 0.1 mg 405.6 EUR Description: ANXA1 PeptideAnnexin A7 Protein
20-abx261405 Abbexa
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- 1 mg
- 20 ug
- 5 ug
Annexin A8 Protein
20-abx261421 Abbexa
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- 1 mg
- 20 ug
- 5 ug
Annexin A4 Protein
20-abx262872 Abbexa
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- Ask for price
- 10 ug
- 1 mg
- 2 µg
Annexin A6 Protein
20-abx262873 Abbexa
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- Ask for price
- Ask for price
- 10 ug
- 1 mg
- 2 µg
Annexin A5 Protein
20-abx260555 Abbexa
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- Ask for price
- Ask for price
- 1 mg
- 20 ug
- 5 ug
Annexin A3 Protein
20-abx260563 Abbexa
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- Ask for price
- Ask for price
- 1 mg
- 20 ug
- 5 ug
Annexin A7 Protein
abx261405-1g Abbexa 1 µg 225 EUR Annexin A7 Protein
abx261405-50g Abbexa 50 µg 3550 EUR Annexin A7 Protein
abx261405-5g Abbexa 5 µg 325 EUR Annexin A8 Protein
abx261421-1g Abbexa 1 µg 225 EUR Annexin A8 Protein
abx261421-50g Abbexa 50 µg 3550 EUR Annexin A8 Protein
abx261421-5g Abbexa 5 µg 325 EUR Annexin A6 Protein
abx262873-10mg Abbexa 10 mg 325 EUR Annexin A6 Protein
abx262873-25mg Abbexa 25 mg 6575 EUR Annexin A6 Protein
abx262873-5mg Abbexa 5 mg 225 EUR Annexin A6 Antibody
8C0126 AAT Bioquest 50ug 368 EUR Description: Annexin A6 AntibodyAnnexin I antibody
10-2468 Fitzgerald 250 ug 590.4 EUR Description: Mouse monoclonal Annexin I antibodyAnnexin I antibody
10-2469 Fitzgerald 250 ug 590.4 EUR Description: Mouse monoclonal Annexin I antibodyAnnexin 1 antibody
10R-11422 Fitzgerald 100 ul 365 EUR Description: Mouse Monoclonal Annexin 1 antibodyAnnexin V antibody
10R-7939 Fitzgerald 100 ug 444 EUR Description: Mouse monoclonal Annexin V antibodyAnnexin VI Antibody
21672 SAB 100ul 319 EUR Annexin VI Antibody
21672-100ul SAB 100ul 302.4 EUR Annexin VI Antibody
21672-50ul SAB 50ul 224.4 EUR Annexin V antibody
20R-1522 Fitzgerald 100 ug 781.2 EUR Description: Rabbit polyclonal Annexin V antibodyAnnexin A1 antibody
22818 SAB 100ul 479 EUR Annexin A1 antibody
22818-100ul SAB 100ul 468 EUR Annexin A6 Antibody
33322 SAB 100ul 319 EUR
Collectively, these data suggest that MEHP induces oxidative stress by disrupting the activities of antioxidant enzymes. This may lead to decreased expression of cell-cycle regulators and antiapoptotic regulators and increased expression of proapoptotic factors, which then may lead to inhibition of follicle growth.