Mono-(2-ethylhexyl) phthalate induces oxidative stress and inhibits growth of mouse ovarian antral follicles.

Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of the most commonly used plasticizer, di-(2-ethylhexyl) phthalate, and is considered to be a reproductive toxicant. However, little is known about the effects of MEHP on ovarian antral follicles.

Thus, the present study tested the hypothesis that MEHP inhibits follicle growth via oxidative stress pathways. The data indicate that MEHP increases reactive oxygen species (ROS) levels and inhibits follicle growth in antral follicles, whereas N-acetylcysteine (NAC; an antioxidant) restores ROS levels to control levels and rescues follicles from MEHP-induced inhibition of follicle growth.

To further analyze the mechanism by which MEHP induces oxidative stress and inhibits follicle growth, the expression and activities of various key antioxidant enzymes (copper/zinc superoxide dismutase [SOD1], glutathione peroxidase [GPX], and catalase [CAT]) and the expression of key cell-cycle regulators (Ccnd2, Ccne1, and Cdk4) and apoptotic regulators (Bcl-2 and Bax) were compared in control and MEHP-treated follicles.

The data indicate that MEHP inhibits the expression and activities of SOD1 and GPX; does not inhibit Cat expression; inhibits the expression of Ccnd2, Ccne1, Cdk4, and Bcl-2; but increases the expression of Bax compared to controls. Furthermore, NAC blocks these toxic effects of MEHP.

Rat annexin 5, ANX-5 GENLISA ELISA

KLR0457 Krishgen 1 x 96 wells 341 EUR

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-1mg

QP8982-ye-1mg EnQuireBio 1mg 3296.4 EUR

Annexin A5 (Annexin V)-Biotin

MC-252 Kamiya Biomedical Company VAA-33 Ask for price

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-10ug

QP8982-ye-10ug EnQuireBio 10ug 369.6 EUR

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-50ug

QP8982-ye-50ug EnQuireBio 50ug 500.4 EUR

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-100ug

QP8982-ye-100ug EnQuireBio 100ug 804 EUR

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-200ug

QP8982-ye-200ug EnQuireBio 200ug 1282.8 EUR

Recombinant Human ANXA5/ Annexin 5/ Annexin A5 Protein, His, Yeast-500ug

QP8982-ye-500ug EnQuireBio 500ug 2164.8 EUR

Mouse annexin 5, ANX-5 GENLISA ELISA    

KLM0502 Krishgen 1 x 96 wells 341 EUR

Human Annexin 5, ANX-5 GENLISA ELISA

KBH1107 Krishgen 1 x 96 wells 286 EUR

Bovine Annexin 5, ANX-5 GENLISA ELISA

KLB0304 Krishgen 1 x 96 wells 341 EUR

Annexin VII (Annexin VII) Antibody

abx230439-100ug Abbexa 100 ug 610.8 EUR

Annexin VIII (Annexin VIII) Antibody

abx230440-100ug Abbexa 100 ug 577.2 EUR

Annexin A4/Annexin IV Rabbit mAb

A9203 Abclonal 50μL 1557.4 EUR
Description: A synthetic peptide of human CD62P/P-selectin.

Annexin A5/Annexin V Rabbit mAb

A9262 Abclonal 200μL 213.2 EUR
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1).

Annexin VI/Annexin A6 Rabbit pAb

E2382616 EnoGene 100ul 225 EUR
Description: Available in various conjugation types.

Annexin A6/Annexin VI Rabbit mAb

A22038 Abclonal 500μL 83.2 EUR
Description: Recombinant protein of Human PMF1.

Annexin A5 (Annexin V) (VAC-alpha)

MC-250 Kamiya Biomedical Company VAA-33 Ask for price

Horse ANX5(Annexin 5) ELISA Kit

EHS0047 FN Test 96T 681.12 EUR
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Horse;Sensitivity: 0.938 ng/ml

Rat Annexin A5(Annexin A5) ELISA Kit

NSL0938r Sunlong 96 Tests 498 EUR

Goat Annexin A5(Annexin A5) ELISA Kit

NSL1380Gt Sunlong 96T 528 EUR

Human Annexin A5(Annexin A5) ELISA Kit

NSL1060Hu Sunlong 96 wells 468 EUR

Canine Annexin A5(Annexin A5) ELISA Kit

NSL1765Ca Sunlong 1860 wells 528 EUR

Bovine Annexin A5(Annexin A5) ELISA Kit

NSL1870Bo Sunlong 96 T 528 EUR

Porcine Annexin A5(Annexin A5) ELISA Kit

NSL1451Po Sunlong 96T 528 EUR

Chicken Annexin A5(Annexin A5) ELISA Kit

NSL1507Ch Sunlong 1602 T 528 EUR

Guinea pig Annexin A5(Annexin A5) ELISA Kit

NSL1474Gp Sunlong 96T 528 EUR

Annexin V 647

E16FXP023a-100T EnoGene 100 tests 433.33 EUR
Description: Available in various conjugation types.

Annexin A3

E8ER1802-50 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A1

E8ER1803-70 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A7

E8ER1903-73 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin V

E8ET1702-62 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A2

E8ET1704-49 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin IV

E8ET7108-56 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A2

AP89346 SAB 1mg 2640 EUR

Annexin A5

AP78464 SAB 1mg 2640 EUR

Annexin A1

AP78480 SAB 1mg 2640 EUR

Annexin A2

AP79238 SAB 1mg 2640 EUR

Annexin A2

AP80284 SAB 1mg 2640 EUR

Annexin A6

AP80377 SAB 1mg 2640 EUR

Annexin A5

AP80382 SAB 1mg 2640 EUR

Annexin A1

AP80399 SAB 1mg 2640 EUR

Annexin A6

AP80426 SAB 1mg 2640 EUR

Annexin A1

AP80428 SAB 1mg 2640 EUR

Annexin A3

AP80504 SAB 1mg 2640 EUR

Annexin A4

AP80514 SAB 1mg 2640 EUR

Annexin A5

AP80515 SAB 1mg 2640 EUR

Annexin A6

AP80516 SAB 1mg 2640 EUR

Annexin A1

AP80543 SAB 1mg 2640 EUR

Annexin A6

AP80574 SAB 1mg 2640 EUR

Annexin A3

AP80584 SAB 1mg 2640 EUR

Annexin A4

AP80585 SAB 1mg 2640 EUR

Annexin A5

AP80586 SAB 1mg 2640 EUR

Annexin A1

AP80590 SAB 1mg 2640 EUR

Annexin A4

AP80619 SAB 1mg 2640 EUR

Annexin A6

AP80630 SAB 1mg 2640 EUR

Annexin A3

AP80655 SAB 1mg 2640 EUR

Annexin A4

AP80657 SAB 1mg 2640 EUR

Annexin A3

AP80658 SAB 1mg 2640 EUR

Annexin A5

AP80659 SAB 1mg 2640 EUR

Annexin A1

AP80687 SAB 1mg 2640 EUR

Annexin A2

AP80694 SAB 1mg 2640 EUR

Annexin A2

AP80710 SAB 1mg 2640 EUR

Annexin A2

AP80745 SAB 1mg 2640 EUR

Annexin A4

AP80760 SAB 1mg 2640 EUR

Annexin A2

AP80800 SAB 1mg 2640 EUR

Annexin A4

AP80952 SAB 1mg 2640 EUR

Annexin V

Annv Clemente Associates LLC 2 ML 367.5 EUR

Annexin V

ANXVCFB-200T ImmunoStep 200 test 350.9 EUR
Description: Annexin V conjugate in the selected fluorochrome

Annexin V

ANXVDY-200T ImmunoStep 200 test 287.1 EUR
Description: Annexin V conjugate in the selected fluorochrome

Annexin V

ANXVF-200T ImmunoStep 200 test 259.6 EUR
Description: Annexin V conjugate in the selected fluorochrome

Annexin V

ANXVPE-200T ImmunoStep 200 test 287.1 EUR
Description: Annexin V conjugate in the selected fluorochrome

Annexin A1

PR27207 Neuromics 2 ug 465.6 EUR

Annexin A2

PR27211 Neuromics 5 ug 229.2 EUR

Annexin A3

PR27212 Neuromics 5 ug 229.2 EUR

Annexin A4

PR27213 Neuromics 2 ug 229.2 EUR

Annexin A5

PR27214 Neuromics 5 ug 229.2 EUR

Annexin A6

PR27215 Neuromics 2 ug 229.2 EUR

Annexin A7

PR27216 Neuromics 5 ug 229.2 EUR

Annexin A8

PR27217 Neuromics 5 ug 229.2 EUR

Annexin V

abx095275-100l Abbexa 100 µl 443.75 EUR

Annexin V

abx095275-1ml Abbexa 1 ml Ask for price

Annexin V

abx095275-200l Abbexa 200 µl Ask for price

Annexin A6

MO22207 Neuromics each 296.1 EUR

Annexin A1

PR27207-B Neuromics each 337.5 EUR

Annexin A2

PR27211-B Neuromics each 184.5 EUR

Annexin A3

PR27212-B Neuromics each 184.5 EUR

Annexin A4

PR27213-B Neuromics each 184.5 EUR

Annexin A5

PR27214-B Neuromics each 184.5 EUR

Annexin A6

PR27215-B Neuromics each 184.5 EUR

Annexin A7

PR27216-B Neuromics each 184.5 EUR

Annexin A8

PR27217-B Neuromics each 184.5 EUR

Annexin A5

RA22139 Neuromics each 272.7 EUR

Annexin A6

RA22143 Neuromics each 296.1 EUR

Annexin Ⅰ

PA1006 Antagene 100μg 260 EUR

Annexin Ⅳ

PA1007 Antagene 100μg 260 EUR

Annexin Ⅴ

PA1008 Antagene 100μg 260 EUR

Annexin A2

PA1348 Antagene 100μg 260 EUR

Annexin Ⅰ

PA1006-S Antagene 100μg 290 EUR
Description: Polyclonal Antibodies Conjugated to Sepharose Beads

Annexin Ⅳ

PA1007-S Antagene 100μg 290 EUR
Description: Polyclonal Antibodies Conjugated to Sepharose Beads

Annexin Ⅴ

PA1008-S Antagene 100μg 290 EUR
Description: Polyclonal Antibodies Conjugated to Sepharose Beads

Annexin A2

PA1348-M Antagene 100μg 290 EUR
Description: Polyclonal Antibodies Conjugated to Magnetic Beads

Annexin A2

PA1348-S Antagene 100μg 290 EUR
Description: Polyclonal Antibodies Conjugated to Sepharose Beads

Annexin A13

E8ER1903-72 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A10

E8ET1610-57 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin A10

AP80493 SAB 1mg 2640 EUR

Annexin A10

AP80951 SAB 1mg 2640 EUR

Annexin A10

PR27208 Neuromics 5 ug 229.2 EUR

Annexin A11

PR27209 Neuromics 5 ug 229.2 EUR

Annexin A13

PR27210 Neuromics 2 ug 229.2 EUR

Annexin A10

PR27208-B Neuromics each 184.5 EUR

Annexin A11

PR27209-B Neuromics each 184.5 EUR

Annexin A13

PR27210-B Neuromics each 184.5 EUR

Rabbit polyclonal antibody to Annexin A1 (annexin A1)

TA308701 Origene Technologies GmbH 100 µl Ask for price

Rabbit polyclonal antibody to Annexin VII (annexin A7)

TA308796 Origene Technologies GmbH 100 µl Ask for price

Recombinant Human ANXA5/Annexin Ⅴ/Annexin A5 Protein

PKSH031601-100ug Elabscience Biotech 100ug 739 EUR
Description: Human

Recombinant Human ANXA5/Annexin Ⅴ/Annexin A5 Protein

PKSH031601-20ug Elabscience Biotech 20ug 249 EUR
Description: Human

OKRC00159-96W - Annexin A5/Annexin V ELISA Kit (Human)

OKRC00159-96W Aviva Systems Biology 96Wells 600 EUR

Annexin Peptide

45-007P ProSci 0.1 mg 405.6 EUR
Description: (NT) Annexin A11 Peptide

Annexin Peptide

45-008P ProSci 0.1 mg 405.6 EUR
Description: (NT) Annexin A2 Peptide

Polyclonal ANXA6/Annexin A6/Annexin VI Antibody (aa1-50)

APR02865G Leading Biology 0.05ml 580.8 EUR
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ANXA6/Annexin A6/Annexin VI (aa1-50). This antibody is tested and proven to work in the following applications:

Annexin V (PE)

abx090613-100tests Abbexa 100 tests 644.4 EUR

Annexin V PE

EXB0027 ExBio 100 tests 198 EUR

Annexin-2 Antibody

39396-100ul SAB 100ul 468 EUR

Annexin-2 Antibody

E300092 EnoGene 100ug/200ul 275 EUR
Description: Available in various conjugation types.

Annexin-2 Antibody

E312231 EnoGene 200ul 275 EUR
Description: Available in various conjugation types.

Annexin V (APC)

abx090615-100tests Abbexa 100 tests 718.8 EUR

Annexin V APC

EXB0028 ExBio 100 tests 198 EUR

Annexin V (APC)

abx090615-100l Abbexa 100 µl 537.5 EUR

Annexin V (APC)

abx090615-1ml Abbexa 1 ml Ask for price

Annexin V (APC)

abx090615-200l Abbexa 200 µl Ask for price

Annexin V, sulforhodamine 101 conjugate (Texas-Red Annexin V)

29002 Biotium 0.5ML 332.4 EUR
Description: Minimum order quantity: 1 unit of 0.5ML

Annexin V (FITC)

abx090611-100tests Abbexa 100 tests 510 EUR

Annexin V- FITC

E16FXP018a-100T EnoGene 100 tests 400 EUR
Description: Available in various conjugation types.

Annexin V FITC

EXB0024 ExBio 100 tests 165 EUR

Annexin A2/ANXA2

E21-205 EnoGene 10ug 225 EUR
Description: Recombinant Human Annexin A2 is produced by our E.coli expression system and the target gene encoding Ser2-Asp339 is expressed.

Annexin V, Human

E34M143H EnoGene 5 μg 155 EUR

Annexin A3 siRNA

20-abx900342 Abbexa
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  • 15 nmol
  • 30 nmol

Annexin V siRNA

20-abx900343 Abbexa
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  • 15 nmol
  • 30 nmol

Annexin A3 siRNA

20-abx907669 Abbexa
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  • 15 nmol
  • 30 nmol

Annexin A3 siRNA

20-abx907670 Abbexa
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  • 15 nmol
  • 30 nmol

Annexin V siRNA

20-abx907673 Abbexa
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  • 15 nmol
  • 30 nmol

Annexin V siRNA

20-abx907674 Abbexa
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  • Ask for price
  • 15 nmol
  • 30 nmol

ANXA1 (Annexin A1)

MO47023 Neuromics 100 ul 418.8 EUR

Annexin A2 Human

rAP-2789 Angio Proteomie Inquiry Ask for price

Recombinant human Annexin A5/Annexin V/ANXA5 Protein, His Tag

E40KMP1303 EnoGene 20ug 495 EUR

Annexin A3 Protein

abx061599-200ug Abbexa 200 ug 1528.8 EUR

Annexin A1 protein

30R-1291 Fitzgerald 20 ug 492 EUR
Description: Purified recombinant Human Annexin A1 protein

Annexin A5 protein

30R-1406 Fitzgerald 100 ug 267 EUR
Description: Purified recombinant Human Annexin A5 protein

Annexin A3 protein

30R-1453 Fitzgerald 100 ug 267 EUR
Description: Purified recombinant Human Annexin A3 protein

Annexin A4 protein

30R-1463 Fitzgerald 100 ug 622 EUR
Description: Purified recombinant Human Annexin A4 protein

Annexin A5 protein

30R-3209 Fitzgerald 50 ug 308.4 EUR
Description: Purified recombinant Annexin A5 protein

Annexin A1 Peptide

46-785P ProSci 0.1 mg 405.6 EUR
Description: ANXA1 Peptide

Annexin A7 Protein

20-abx261405 Abbexa
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  • 1 mg
  • 20 ug
  • 5 ug

Annexin A8 Protein

20-abx261421 Abbexa
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  • 1 mg
  • 20 ug
  • 5 ug

Annexin A4 Protein

20-abx262872 Abbexa
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  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Annexin A6 Protein

20-abx262873 Abbexa
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  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Annexin A5 Protein

20-abx260555 Abbexa
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  • 1 mg
  • 20 ug
  • 5 ug

Annexin A3 Protein

20-abx260563 Abbexa
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  • 1 mg
  • 20 ug
  • 5 ug

Annexin A7 Protein

abx261405-1g Abbexa 1 µg 225 EUR

Annexin A7 Protein

abx261405-50g Abbexa 50 µg 3550 EUR

Annexin A7 Protein

abx261405-5g Abbexa 5 µg 325 EUR

Annexin A8 Protein

abx261421-1g Abbexa 1 µg 225 EUR

Annexin A8 Protein

abx261421-50g Abbexa 50 µg 3550 EUR

Annexin A8 Protein

abx261421-5g Abbexa 5 µg 325 EUR

Annexin A6 Protein

abx262873-10mg Abbexa 10 mg 325 EUR

Annexin A6 Protein

abx262873-25mg Abbexa 25 mg 6575 EUR

Annexin A6 Protein

abx262873-5mg Abbexa 5 mg 225 EUR

Annexin A6 Antibody

8C0126 AAT Bioquest 50ug 368 EUR
Description: Annexin A6 Antibody

Annexin I antibody

10-2468 Fitzgerald 250 ug 590.4 EUR
Description: Mouse monoclonal Annexin I antibody

Annexin I antibody

10-2469 Fitzgerald 250 ug 590.4 EUR
Description: Mouse monoclonal Annexin I antibody

Annexin 1 antibody

10R-11422 Fitzgerald 100 ul 365 EUR
Description: Mouse Monoclonal Annexin 1 antibody

Annexin V antibody

10R-7939 Fitzgerald 100 ug 444 EUR
Description: Mouse monoclonal Annexin V antibody

Annexin VI Antibody

21672 SAB 100ul 319 EUR

Annexin VI Antibody

21672-100ul SAB 100ul 302.4 EUR

Annexin VI Antibody

21672-50ul SAB 50ul 224.4 EUR

Annexin V antibody

20R-1522 Fitzgerald 100 ug 781.2 EUR
Description: Rabbit polyclonal Annexin V antibody

Annexin A1 antibody

22818 SAB 100ul 479 EUR

Annexin A1 antibody

22818-100ul SAB 100ul 468 EUR

Annexin A6 Antibody

33322 SAB 100ul 319 EUR

Collectively, these data suggest that MEHP induces oxidative stress by disrupting the activities of antioxidant enzymes. This may lead to decreased expression of cell-cycle regulators and antiapoptotic regulators and increased expression of proapoptotic factors, which then may lead to inhibition of follicle growth.