Transcriptome profiling and pathway analysis of the effects of mono-(2-ethylhexyl) phthalate in mouse Sertoli cells.

Phthalates are confirmed to have poisonous effects on the reproductive system and are prone to have additional damaging actions in people. The current examine explored the molecular mechanisms of the poisonous impact of mono-(2-ethylhexyl) phthalate (MEHP) on mouse Sertoli cells.

Cell apoptosis and proliferation assays had been used to evaluate the effects of MEHP on the TM4 Sertoli cell line derived from mouse testes. TM4 cells had been handled with two doses of MEHP or left untreated as a management group, adopted by RNA extraction and analysis utilizing high-throughput transcriptome sequencing know-how. The gene expression profile obtained was then subjected to a bioinformatics analysis to discover the molecular mechanisms of reproductive toxicity.

The outcomes revealed that 528 and 269 genes had been upregulated in the high- and low-dose MEHP teams of cells in contrast with the management group, whereas 148 and 173 genes had been downregulated. Gene ontology (GO) analysis indicated that the otherwise expressed genes had been related with the GO time period ‘extracellular area’ of the mobile part area in the excessive and low MEHP teams.

Annexin V-PE/7-AAD Apoptosis Kit
E-CK-A216-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/7-AAD Apoptosis Kit
E-CK-A216-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/7-AAD Apoptosis Kit
E-CK-A216-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/7-AAD Apoptosis Kit
E-CK-A216-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A218-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A218-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A218-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A218-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/7-AAD Apoptosis Kit
E-CK-A212-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/7-AAD Apoptosis Kit
E-CK-A212-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/7-AAD Apoptosis Kit
E-CK-A212-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/7-AAD Apoptosis Kit
E-CK-A212-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/7-AAD Apoptosis Kit
E-CK-A220-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/7-AAD Apoptosis Kit
E-CK-A220-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/7-AAD Apoptosis Kit
E-CK-A220-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-EGFP/7-AAD Apoptosis Kit
E-CK-A220-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-FITC/7-AAD Apoptosis Kit
K1139-100	ApexBio	100assays	184 EUR
Description: Apoptosis Detection kit|Annexin V Apoptosis Detection Kits
Annexin V-FITC/7-AAD Apoptosis Kit
K1139-20	ApexBio	20assays	57.6 EUR
Description: Apoptosis Detection kit|Annexin V Apoptosis Detection Kits
Annexin V-FITC/7-AAD Apoptosis Kit
K1139-50	ApexBio	50assays	116 EUR
Description: Apoptosis Detection kit|Annexin V Apoptosis Detection Kits
Annexin V -AF555/ 7-AAD apoptosis kit
EGQ0023	EnoGene	50T	225 EUR
Annexin V-AF555/7-AAD apoptosis kit
MBS8584930-50Tests	MyBiosource	50Tests	305 EUR
Annexin V-AF555/7-AAD apoptosis kit
MBS8584930-5x50Tests	MyBiosource	5x50Tests	1480 EUR
Annexin V-Cyanine5/7-AAD Apoptosis Kit
E-CK-A222-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/7-AAD Apoptosis Kit
E-CK-A222-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/7-AAD Apoptosis Kit
E-CK-A222-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/7-AAD Apoptosis Kit
E-CK-A222-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V (PE) / 7-AAD Apoptosis Detection Kit (PE)
abx090609-100tests	Abbexa	100 tests	718.8 EUR
Annexin A5 (Annexin V)-FITC
MC-251	Kamiya Biomedical Company	VAA-33	Ask for price
Annexin A5 (Annexin V)-Biotin
MC-252	Kamiya Biomedical Company	VAA-33	Ask for price
Annexin V
Annv	Clemente Associates LLC	2 ML	367.5 EUR
Annexin V
ANXVCFB-200T	ImmunoStep	200 test	350.9 EUR
Description: Annexin V conjugate in the selected fluorochrome
Annexin V
ANXVDY-200T	ImmunoStep	200 test	287.1 EUR
Description: Annexin V conjugate in the selected fluorochrome
Annexin V
ANXVF-200T	ImmunoStep	200 test	259.6 EUR
Description: Annexin V conjugate in the selected fluorochrome
Annexin V
ANXVPE-200T	ImmunoStep	200 test	287.1 EUR
Description: Annexin V conjugate in the selected fluorochrome
Annexin V
abx095275-100l	Abbexa	100 µl	443.75 EUR
Annexin V
abx095275-1ml	Abbexa	1 ml	Ask for price
Annexin V
abx095275-200l	Abbexa	200 µl	Ask for price
Annexin V
E8ET1702-62	EnoGene	100ul	275 EUR
Description: Available in various conjugation types.
Annexin V
MBS1802274-01mg	MyBiosource	0.1mg	305 EUR
Annexin V
MBS1802274-5x01mg	MyBiosource	5x0.1mg	1120 EUR
Annexin V
MBS8534369-01mL	MyBiosource	0.1mL	345 EUR
Annexin V
MBS8534369-01mLAF405L	MyBiosource	0.1mL(AF405L)	565 EUR
Annexin V
MBS8534369-01mLAF405S	MyBiosource	0.1mL(AF405S)	565 EUR
Annexin V
MBS8534369-01mLAF610	MyBiosource	0.1mL(AF610)	565 EUR
Annexin V
MBS8534369-01mLAF635	MyBiosource	0.1mL(AF635)	565 EUR
Annexin V-PE/Cyanine7/7-AAD Apoptosis Kit
E-CK-A228-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/Cyanine7/7-AAD Apoptosis Kit
E-CK-A228-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/Cyanine7/7-AAD Apoptosis Kit
E-CK-A228-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/Cyanine7/7-AAD Apoptosis Kit
E-CK-A228-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-00-10rxns	Vazyme	10 rxns	1.09 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-01	Vazyme	50 rxn	96.5 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-01-50rxns	Vazyme	50 rxns	100.28 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-02	Vazyme	100 rxn	145 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-02-100rxns	Vazyme	100 rxns	150.42 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
DLSM0246	DL Develop	100 Assays	Ask for price
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.
Annexin V-APC/Cyanine7/7-AAD Apoptosis Kit
E-CK-A230-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/Cyanine7/7-AAD Apoptosis Kit
E-CK-A230-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/Cyanine7/7-AAD Apoptosis Kit
E-CK-A230-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-APC/Cyanine7/7-AAD Apoptosis Kit
E-CK-A230-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V (APC) / 7-AAD Apoptosis Detection Kit
abx090607-100l	Abbexa	100 µl	587.5 EUR
Annexin V (APC) / 7-AAD Apoptosis Detection Kit
abx090607-1ml	Abbexa	1 ml	Ask for price
Annexin V (APC) / 7-AAD Apoptosis Detection Kit
abx090607-200l	Abbexa	200 µl	Ask for price
Annexin V-PE/7-AAD Apoptosis Detection Kit
E28LS4004-100	EnoGene	100T	793.65 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
E28LS4004-20	EnoGene	20T	279.37 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
E28LS4004-50	EnoGene	50T	507.94 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS355857-100Tests	MyBiosource	100Tests	560 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS355857-5x100Tests	MyBiosource	5x100Tests	2525 EUR
Annexin V-PE / 7-AAD Apoptosis Detection Kit
MBS355218-100Tests	MyBiosource	100Tests	560 EUR
Annexin V-PE / 7-AAD Apoptosis Detection Kit
MBS355218-25Tests	MyBiosource	25Tests	335 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS2557018-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS2557018-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS2557018-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS2557018-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS2557018-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS8305410-100Assays	MyBiosource	100Assays	420 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS8305410-50Assays	MyBiosource	50Assays	365 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
MBS8305410-5x100Assays	MyBiosource	5x100Assays	1830 EUR
Annexin V-PE/7-AAD Apoptosis Detection Kit
RDSM246	Reddot Biotech	100 Assays	350 EUR
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.
Annexin V (FITC) / 7-AAD Apoptosis Detection Kit
abx090603-100tests	Abbexa	100 tests	594 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS355856-100Tests	MyBiosource	100Tests	620 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS355856-5x100Tests	MyBiosource	5x100Tests	2795 EUR
Annexin V-APC / 7-AAD Apoptosis Detection Kit
MBS355215-100Tests	MyBiosource	100Tests	620 EUR
Annexin V-APC / 7-AAD Apoptosis Detection Kit
MBS355215-25Tests	MyBiosource	25Tests	360 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS2557020-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS2557020-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS2557020-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS2557020-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-APC/7-AAD Apoptosis Detection Kit
MBS2557020-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS355854-100Tests	MyBiosource	100Tests	455 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS355854-5x100Tests	MyBiosource	5x100Tests	2050 EUR
Annexin V-FITC / 7-AAD Apoptosis Detection Kit
MBS355222-100Tests	MyBiosource	100Tests	455 EUR
Annexin V-FITC / 7-AAD Apoptosis Detection Kit
MBS355222-25Tests	MyBiosource	25Tests	295 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS2557015-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS2557015-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS2557015-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS2557015-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-FITC/7-AAD Apoptosis Detection Kit
MBS2557015-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-EGFP/7-AAD Apoptosis Detection Kit
MBS2557022-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-EGFP/7-AAD Apoptosis Detection Kit
MBS2557022-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-EGFP/7-AAD Apoptosis Detection Kit
MBS2557022-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-EGFP/7-AAD Apoptosis Detection Kit
MBS2557022-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-EGFP/7-AAD Apoptosis Detection Kit
MBS2557022-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-EV450/7-AAD Apoptosis Detection Kit
MBS2567088-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-EV450/7-AAD Apoptosis Detection Kit
MBS2567088-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-EV450/7-AAD Apoptosis Detection Kit
MBS2567088-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-EV450/7-AAD Apoptosis Detection Kit
MBS2567088-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-EV450/7-AAD Apoptosis Detection Kit
MBS2567088-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-EV500/7-AAD Apoptosis Detection Kit
MBS2567090-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-EV500/7-AAD Apoptosis Detection Kit
MBS2567090-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-EV500/7-AAD Apoptosis Detection Kit
MBS2567090-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-EV500/7-AAD Apoptosis Detection Kit
MBS2567090-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-EV500/7-AAD Apoptosis Detection Kit
MBS2567090-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-AF488/7-AAD Apoptosis Detection Kit
MBS2567092-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-AF488/7-AAD Apoptosis Detection Kit
MBS2567092-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-AF488/7-AAD Apoptosis Detection Kit
MBS2567092-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-AF488/7-AAD Apoptosis Detection Kit
MBS2567092-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-AF488/7-AAD Apoptosis Detection Kit
MBS2567092-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V-AF647/7-AAD Apoptosis Detection Kit
MBS2557017-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-AF647/7-AAD Apoptosis Detection Kit
MBS2557017-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-AF647/7-AAD Apoptosis Detection Kit
MBS2557017-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-AF647/7-AAD Apoptosis Detection Kit
MBS2557017-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-AF647/7-AAD Apoptosis Detection Kit
MBS2557017-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V 647
E16FXP023a-100T	EnoGene	100 tests	433.33 EUR
Description: Available in various conjugation types.
Annexin A5/Annexin V Rabbit pAb
A13945	Abclonal	200μL	1246.96 EUR
Annexin A5/Annexin V Rabbit mAb
A9262	Abclonal	200μL	343.62 EUR
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1).
Annexin A5/Annexin V Rabbit pAb
E45R20506N	EnoGene	50 ul	297.75 EUR
Annexin A5/Annexin V Rabbit mAb
E45R12113N	EnoGene	50 ul	346.5 EUR
Annexin V-Elab Fluor® 647/7-AAD Apoptosis Kit
E-CK-A214-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 647/7-AAD Apoptosis Kit
E-CK-A214-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 647/7-AAD Apoptosis Kit
E-CK-A214-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 647/7-AAD Apoptosis Kit
E-CK-A214-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 488/7-AAD Apoptosis Kit
E-CK-A238-100Assays	Elabscience Biotech	100 Assays	260 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 488/7-AAD Apoptosis Kit
E-CK-A238-200Assays	Elabscience Biotech	200 Assays	398 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 488/7-AAD Apoptosis Kit
E-CK-A238-20Assays	Elabscience Biotech	20 Assays	75 EUR
Description: Apoptosis/Annexin V
Annexin V-Elab Fluor® 488/7-AAD Apoptosis Kit
E-CK-A238-50Assays	Elabscience Biotech	50 Assays	150 EUR
Description: Apoptosis/Annexin V
Annexin A5 (Annexin V) (VAC-alpha)
MC-250	Kamiya Biomedical Company	VAA-33	Ask for price
Annexin V-Cyanine5/7-AAD Apoptosis Detection Kit
MBS2567083-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-Cyanine5/7-AAD Apoptosis Detection Kit
MBS2567083-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-Cyanine5/7-AAD Apoptosis Detection Kit
MBS2567083-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-Cyanine5/7-AAD Apoptosis Detection Kit
MBS2567083-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-Cyanine5/7-AAD Apoptosis Detection Kit
MBS2567083-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin V (APC) / 7-AAD Apoptosis Detection Kit (APC)
abx090607-100tests	Abbexa	100 tests	811.2 EUR
Annexin V-ER780 / 7-AAD Apoptosis Detection Kit [A240]
MBS2567759-100Assays	MyBiosource	100Assays	310 EUR
Annexin V-ER780 / 7-AAD Apoptosis Detection Kit [A240]
MBS2567759-200Assays	MyBiosource	200Assays	395 EUR
Annexin V-ER780 / 7-AAD Apoptosis Detection Kit [A240]
MBS2567759-20Assays	MyBiosource	20Assays	195 EUR
Annexin V-ER780 / 7-AAD Apoptosis Detection Kit [A240]
MBS2567759-50Assays	MyBiosource	50Assays	240 EUR
Annexin V-ER780 / 7-AAD Apoptosis Detection Kit [A240]
MBS2567759-5x200Assays	MyBiosource	5x200Assays	1800 EUR
Annexin A5/Annexin V polyclonal antibody
BS75535	Bioworld Biotech	50ul	358 EUR
Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Annexin V-iFluor 594 + 7-AAD Apoptosis Detection Reagent
E34M002D	EnoGene	50 rxn	225 EUR
Annexin V-iFluor 594 + 7-AAD Apoptosis Detection Reagent
MBS8579597-50Reactions	MyBiosource	50Reactions	305 EUR
Annexin V-iFluor 594 + 7-AAD Apoptosis Detection Reagent
MBS8579597-5x50Reactions	MyBiosource	5x50Reactions	1220 EUR
Annexin V (PE)
abx090613-100tests	Abbexa	100 tests	644.4 EUR
Annexin V PE
EXB0027	ExBio	100 tests	198 EUR
Annexin V PE
MBS1802118-100Tests	MyBiosource	100Tests	305 EUR
Annexin V PE
MBS1802118-5x100Tests	MyBiosource	5x100Tests	1120 EUR
Annexin V (PE)
MBS6251357-01mL	MyBiosource	0.1(mL	860 EUR
Annexin V (PE)
MBS6251357-5x01mL	MyBiosource	5x0.1mL	3720 EUR
Annexin V (AP)
MBS6247389-01mL	MyBiosource	0.1(mL	860 EUR
Annexin V (AP)
MBS6247389-5x01mL	MyBiosource	5x0.1mL	3720 EUR
Annexin V PE
MBS697175-100Tests	MyBiosource	100Tests	300 EUR
Annexin V PE
MBS697175-25Tests	MyBiosource	25Tests	185 EUR

Compared with the management group, eight widespread pathway adjustments had been recognized in the high- and low-dose MEHP teams, together with ‘terpenoid spine biosynthesis’. Reverse transcription-quantitative polymerase chain response analysis was used to validation, and airtight effects had been noticed for sure genes. These outcomes present an essential foundation and experimental information for additional analysis into the mechanisms of phthalate-induced toxicity.

Mono-butyl phthalate-induced mouse testis damage is related to oxidative stress and down-regulated expression of Sox9 and Dazl.

Mono-butyl phthalate (MBP) has reproductive toxicity however the associated mechanisms haven’t been totally elucidated in vivo. We uncovered male Balb/c mice to MBP by gavage at doses of 0, 25, 50, 100, 200 mg/kg for 14 days, and then evaluated the testicular alterations at the histological and molecular ranges. MBP diminished mouse sperm rely together with sperm malformation and seminiferous tubule degeneration in a dose-dependent method.

MBP dosed at 200 mg/kg considerably elevated reactive oxygen species and malondialdehyde content material in mouse testes. High doses of MBP (200 mg/kg) additionally considerably diminished mRNA expressions of testis development and perform associated genes (Sox9 and Dazl). Our findings recommend that oxidative stress and down-regulated expression of Sox9 and Dazl might play essential roles in MBP-induced testis damage.

Mono-2-ethylhexyl phthalate stimulates androgen manufacturing however suppresses mitochondrial perform in mouse leydig cells with completely different steroidogenic potential.

Numerous research have reported on testicular toxicity of phthalates in completely different experimental paradigms and confirmed that Leydig cells (LCs) had been one of the foremost targets of phthalate actions. Adverse effects of phthalates on LCs steroidogenesis have been attributed to their metabolites, monophthalates.

This examine focuses on investigation whether or not LCs responsiveness to monophthalates motion is related to their potential to supply androgens. We discovered that of three monophthalates investigated [ie, mono-2-ethylhexyl phthalate (MEHP), mono-n-butyl phthalate, and mono-n-benzyl phthalate] solely MEHP brought about organic effects on the mouse LCs perform.

This monophthalate stimulated basal steroidogenesis related to upregulation of StAR protein expression with no impact on hCG-stimulated androgen manufacturing by LCs from CBA/Lac and C57BL/6j mouse genotypes had been noticed. Further, MEHP attenuated ATP manufacturing and elevated superoxide technology by each phenotypes of mouse LCs that indicated on mitochondrial dysfunction induced by the monophthalate.

All collectively, our information point out that MEHP-mediated stimulation of steroidogenesis and perturbation in mitochondrial perform usually are not related to the capability of the LCs to synthesize androgens. We recommend that this impact of MEHP noticed in LCs of rodent origin must be considered in analysis of earlier begin of puberty in boys and might spotlight a potential affect of phthalates on reproductive well being in males.