Mono(2-ethylhexyl) phthalate accelerates early folliculogenesis and inhibits steroidogenesis in cultured mouse whole ovaries and antral follicles.
Humans are ubiquitously uncovered to di(2-ethylhexyl) phthalate (DEHP), which is an environmental toxicant current in frequent client merchandise. DEHP doubtlessly targets the ovary via its metabolite mono(2-ethylhexyl) phthalate (MEHP). However, the direct results of MEHP on ovarian folliculogenesis and steroidogenesis, two processes important for reproductive and nonreproductive well being, are unknown.
The current research examined the hypotheses that MEHP instantly accelerates early folliculogenesis through overactivation of phosphatidylinositol 3-kinase (PI3K) signaling, a pathway that regulates primordial follicle quiescence and activation, and inhibits the synthesis of steroid hormones by lowering steroidogenic enzyme ranges. Neonatal ovaries from CD-1 mice have been cultured for six days with car management, DEHP, or MEHP (0.2-20 μg/ml) to evaluate the direct results on folliculogenesis and PI3K signaling. Further, antral follicles from grownup CD-1 mice have been cultured with car management or MEHP (0.1-10 μg/ml) for 24-96 h to ascertain the temporal results of MEHP on steroid hormones and steroidogenic enzymes. In the neonatal ovaries, MEHP, however not DEHP, decreased phosphatase and tensin homolog ranges and elevated phosphorylated protein kinase B ranges, resulting in a lower in the share of germ cells and a rise in the share of main follicles.
In the antral follicles, MEHP decreased the mRNA ranges of 17alpha-hydroxylase-17,20-desmolase, 17beta-hydroxysteroid dehydrogenase, and aromatase resulting in a lower in testosterone, estrone, and estradiol ranges. Collectively, MEHP mediates the impact of DEHP on accelerated folliculogenesis through overactivating PI3K signaling and inhibits steroidogenesis by lowering steroidogenic enzyme ranges.
ANNEXIN V:FITC ASSAY KIT (OKSA11265) |
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| OKSA11265 | Aviva Systems Biology | 300 Tests | 1375.2 EUR |
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Description: Description of target: ANNEXIN V:FITC ASSAY KIT;Species reactivity: Human;Application: FC;Assay info: ;Sensitivity: |
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ANNEXIN V:Biotin ASSAY KIT (OKSA11262) |
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| OKSA11262 | Aviva Systems Biology | 100 Tests | 944.4 EUR |
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Description: Description of target: ANNEXIN V:Biotin ASSAY KIT;Species reactivity: Human;Application: FC;Assay info: ;Sensitivity: |
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ANNEXIN V:Biotin ASSAY KIT (OKSA11264) |
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| OKSA11264 | Aviva Systems Biology | 300 Tests | 1375.2 EUR |
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Description: Description of target: ANNEXIN V:Biotin ASSAY KIT;Species reactivity: Human;Application: FC;Assay info: ;Sensitivity: |
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Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF594 Annexin V (50 assays) |
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| 30067 | Biotium | 1KIT | 514.8 EUR |
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Description: Minimum order quantity: 1 unit of 1KIT |
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Annexin V, R-phycoerythrin conjugate (100 assays) |
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| 9-29045 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uL |
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Annexin V, Allophycocyanin crosslinked conjugate (100 assays) |
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| 9-29057 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uL |
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Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* |
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| 22828 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V (PE) |
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| abx090613-100tests | Abbexa | 100 tests | 644.4 EUR |
Annexin V PE |
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| EXB0027 | ExBio | 100 tests | 244.8 EUR |
Annexin V (APC) |
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| abx090615-100tests | Abbexa | 100 tests | 718.8 EUR |
Annexin V APC |
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| EXB0028 | ExBio | 100 tests | 244.8 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* |
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| 22829 | AAT Bioquest | 100 Tests | 222 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm* |
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| 22830 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V (FITC) |
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| abx090611-100tests | Abbexa | 100 tests | 510 EUR |
Annexin V FITC |
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| EXB0024 | ExBio | 100 tests | 244.8 EUR |
Annexin V, CF770 |
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| 29046 | Biotium | 25ug | 410.4 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V, CF790 |
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| 29047 | Biotium | 25ug | 439.2 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V siRNA |
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| 20-abx900343 | Abbexa |
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Annexin V siRNA |
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| 20-abx907673 | Abbexa |
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Annexin V siRNA |
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| 20-abx907674 | Abbexa |
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Annexin V antibody |
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| 70R-11770 | Fitzgerald | 100 ug | 632.4 EUR |
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Description: Rabbit polyclonal Annexin V antibody |
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Annexin V antibody |
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| 70R-13691 | Fitzgerald | 100 ug | 386.4 EUR |
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Description: Affinity purified Rabbit polyclonal Annexin V antibody |
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Annexin V Antibody |
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| 49376-100ul | SAB | 100ul | 399.6 EUR |
Annexin V Antibody |
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| 49376-50ul | SAB | 50ul | 286.8 EUR |
Annexin V antibody |
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| 10R-7939 | Fitzgerald | 100 ug | 516 EUR |
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Description: Mouse monoclonal Annexin V antibody |
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Annexin V antibody |
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| 20R-1522 | Fitzgerald | 100 ug | 781.2 EUR |
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Description: Rabbit polyclonal Annexin V antibody |
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Annexin V Antibody |
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| 3357-100 | Biovision | each | 464.4 EUR |
Annexin V Antibody |
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| 3357-30T | Biovision | each | 175.2 EUR |
Annexin V Antibody |
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| DF6580 | Affbiotech | 200ul | 420 EUR |
Annexin V Antibody |
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| F49706-0.08ML | NSJ Bioreagents | 0.08 ml | 140.25 EUR |
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Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. |
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Annexin V Antibody |
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| F49706-0.4ML | NSJ Bioreagents | 0.4 ml | 322.15 EUR |
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Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. |
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Annexin V Antibody |
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| F49707-0.08ML | NSJ Bioreagents | 0.08 ml | 140.25 EUR |
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Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt] |
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Annexin V Antibody |
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| F49707-0.4ML | NSJ Bioreagents | 0.4 ml | 322.15 EUR |
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Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt] |
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Annexin V antibody |
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| PAab09988 | Lifescience Market | 100 ug | 463.2 EUR |
Annexin V Antibody |
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| RQ5261 | NSJ Bioreagents | 100ul | 356.15 EUR |
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Description: The protein encoded by the ANXA5 gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. [RefSeq] |
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Annexin V Antibody |
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| R30176 | NSJ Bioreagents | 100 ug | 356.15 EUR |
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Description: Annexin V is also know as endonnexin II (ENX2),or placental protein 4 (PP4). It is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. Annexin V has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. It is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
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Annexin V Antibody |
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| R31475 | NSJ Bioreagents | 100 ug | 356.15 EUR |
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Description: Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
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Annexin V, sulforhodamine 101 conjugate (Texas-Red Annexin V) |
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| 29002 | Biotium | 0.5ML | 332.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, Recombinant |
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| 20014 | AAT Bioquest | 100 ug | 222 EUR |
Annexin V, Recombinant |
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| 20015 | AAT Bioquest | 1 mg | 1509 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry* |
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| 22826 | AAT Bioquest | 100 Tests | 222 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* |
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| 22824 | AAT Bioquest | 100 Tests | 222 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* |
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| 22825 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V ELISA kit |
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| 55R-IB49646 | Fitzgerald | 96 wells | 1413.6 EUR |
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Description: ELISA kit for the detection of Annexin V in the research laboratory |
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Annexin V ELISA kit |
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| 55R-ORG643 | Fitzgerald | 96 wells | 541.2 EUR |
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Description: ELISA kit for the detection of Annexin V in the research laboratory |
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Annexin V Dyomics 647 |
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| EXB0023 | ExBio | 100 tests | 266.4 EUR |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry* |
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| 22827 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V-PE Reagent |
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| 1014-1000 | Biovision | each | 1358.4 EUR |
Annexin V-PE Reagent |
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| 1014-200 | Biovision | each | 483.6 EUR |
Active Annexin V (ANXA5) |
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| 4-APA259Ra01 | Cloud-Clone |
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Description: Recombinant Rat Annexin V expressed in: E.coli |
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Annexin V-Cy5 Reagent |
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| 1013-1000 | Biovision | each | 1358.4 EUR |
Annexin V-Cy5 Reagent |
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| 1013-200 | Biovision | each | 483.6 EUR |
Annexin V-Cy3 Reagent |
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| 1002-1000 | Biovision | each | 1358.4 EUR |
Annexin V-Cy3 Reagent |
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| 1002-200 | Biovision | each | 483.6 EUR |
Annexin V, FITC Labeled |
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| 20030 | AAT Bioquest | 100 tests | 222 EUR |
Annexin V-FITC Reagent |
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| 1001-1000 | Biovision | each | 1495.2 EUR |
Annexin V-FITC Reagent |
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| 1001-200 | Biovision | each | 529.2 EUR |
Annexin V-EGFP Reagent |
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| 1004-1000 | Biovision | each | 1358.4 EUR |
Annexin V-EGFP Reagent |
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| 1004-200 | Biovision | each | 483.6 EUR |
anti- Annexin V antibody |
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| FNab09988 | FN Test | 100µg | 658.5 EUR |
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Description: Antibody raised against Annexin V |
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anti- Annexin V antibody |
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| FNab00436 | FN Test | 100µg | 606.3 EUR |
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Description: Antibody raised against Annexin V |
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anti- Annexin V antibody |
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| FNab00437 | FN Test | 100µg | 606.3 EUR |
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Description: Antibody raised against Annexin V |
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Recombinant human Annexin A5/Annexin V/ANXA5 Protein |
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| RP01180 | Abclonal | 10 μg | 186 EUR |
Annexin V, FITC conjugate |
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| 29001 | Biotium | 0.5ML | 332.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V (ANXA5) Antibody |
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| abx230436-100ug | Abbexa | 100 ug | 577.2 EUR |
Annexin V (ANXA5) Antibody |
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| abx230437-100ug | Abbexa | 100 ug | 577.2 EUR |
Annexin V (ANXA5) Antibody |
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| 20-abx109257 | Abbexa |
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Annexin V (ANXA5) Antibody |
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| 20-abx109540 | Abbexa |
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Annexin V-Cy3 conjugate |
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| 20065 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V-Cy5 conjugate |
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| 20066 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V-Cy5.5 conjugate |
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| 20067 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V-Cy7 conjugate |
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| 20068 | AAT Bioquest | 100 Tests | 222 EUR |
Annexin V (ANXA5) Antibody |
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| abx037408-100ug | Abbexa | 100 ug | 469.2 EUR |
Annexin V (ANXA5) Antibody |
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| abx038319-100ug | Abbexa | 100 ug | 469.2 EUR |
Annexin V (ANXA5) Antibody |
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| 20-abx001443 | Abbexa |
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Annexin V Binding Buffer |
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| abx090621-100tests | Abbexa | 100 tests | 343.2 EUR |
Annexin V (ANXA5) Antibody |
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| abx412125-01mg | Abbexa | 0.1 mg | 1078.8 EUR |
Annexin V (ANXA5) Antibody |
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| abx414511-01mg | Abbexa | 0.1 mg | 526.8 EUR |
Annexin V (ANXA5) Antibody |
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| 20-abx110998 | Abbexa |
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Annexin V (ANXA5) Antibody |
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| 20-abx121016 | Abbexa |
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Annexin V (ANXA5) Antibody |
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| abx032878-400ul | Abbexa | 400 ul | 627.6 EUR |
Annexin V (ANXA5) Antibody |
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| abx032878-80l | Abbexa | 80 µl | 343.2 EUR |
Annexin V (ANXA5) Antibody |
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| abx032879-400ul | Abbexa | 400 ul | 627.6 EUR |
Annexin V (ANXA5) Antibody |
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| abx032879-80l | Abbexa | 80 µl | 343.2 EUR |
Annexin V, CF647 conjugate |
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| 29003 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, CF555 conjugate |
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| 29004 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, CF750 conjugate |
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| 29006 | Biotium | 25ug | 410.4 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V, CF680 conjugate |
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| 29007 | Biotium | 25ug | 410.4 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V, CF633 conjugate |
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| 29008 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, CF568 conjugate |
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| 29010 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, CF594 conjugate |
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| 29011 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, CF350 conjugate |
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| 29012 | Biotium | 0.5mL | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Annexin V, CF800 conjugate |
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| 29078 | Biotium | 25ug | 440.4 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V, CF700 conjugate |
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| 29082 | Biotium | 25ug | 428.4 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V, CF450 conjugate |
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| 29083 | Biotium | 0.5mL | 363.6 EUR |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Annexin V-Biotin Reagent |
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| 1003-100 | Biovision | each | 444 EUR |
Annexin V-Biotin Reagent |
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| 1003-1000 | Biovision | each | 2865.6 EUR |
Annexin V, CF405M conjugate |
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| 29009 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, Biotin conjugate |
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| 29013 | Biotium | 0.5mL | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Annexin V, CF640R conjugate |
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| 29014 | Biotium | 0.5mL | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Annexin V, CF660R conjugate |
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| 29069 | Biotium | 0.5mL | 373.2 EUR |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Annexin V, CF680R conjugate |
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| 29070 | Biotium | 25ug | 412.8 EUR |
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Description: Minimum order quantity: 1 unit of 25ug |
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Annexin V Blocking Peptide |
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| 3357BP-50 | Biovision | each | 183.6 EUR |
Annexin V Blocking Peptide |
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| 33R-10472 | Fitzgerald | 50 ug | 418.8 EUR |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Annexin V antibody, catalog no. 20R-1522 |
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Annexin V Blocking Peptide |
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| 33R-10724 | Fitzgerald | 50 ug | 202.8 EUR |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Annexin V antibody, catalog no. 70R-11770 |
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Annexin V Blocking Peptide |
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| 20-abx161016 | Abbexa |
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Annexin V, CF488A conjugate |
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| 29005 | Biotium | 0.5ML | 368.4 EUR |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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Annexin V, human recombinant |
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| 1005-100 | Biovision | each | 385.2 EUR |
Annexin V, human recombinant |
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| 1005-1000 | Biovision | each | 2440.8 EUR |
Annexin V, human recombinant |
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| 1005-5000 | Biovision | each | 6903.6 EUR |
Annexin V-Biotin conjugate |
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| 20018 | AAT Bioquest | 100 tests | 222 EUR |
Annexin V, PacBlue conjugate |
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| 20089 | AAT Bioquest | 100 tests | 222 EUR |
Annexin V, human recombinant |
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| K2064-.1 | ApexBio | 100 µg | 435.6 EUR |
|
Description: Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin |
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Annexin V, human recombinant |
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| K2064-1 | ApexBio | 1 mg | 2815.2 EUR |
|
Description: Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin |
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Annexin V, human recombinant |
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| K2064-5 | ApexBio | 5 mg | 8402.4 EUR |
|
Description: Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin |
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Recombinant Annexin V (ANXA5) |
|||
| 4-RPA259Hu02 | Cloud-Clone |
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|
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Description: Recombinant Human Annexin V expressed in: E.coli |
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Annexin V Polyclonal Antibody |
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| 27222-100ul | SAB | 100ul | 302.4 EUR |
Annexin V Polyclonal Antibody |
|||
| 27222-50ul | SAB | 50ul | 224.4 EUR |
Annexin V Recombinant Protein |
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| 90-218 | ProSci | 100 ug | 437.1 EUR |
|
Description: Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e. g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the "annexin V affinity assay" to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death. |
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Annexin V Conjugated Antibody |
|||
| C49376 | SAB | 100ul | 476.4 EUR |
Dog Annexin V ELISA kit |
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| E08A0209-192T | BlueGene | 192 tests | 1524 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Annexin V ELISA kit |
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| E08A0209-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Annexin V ELISA kit |
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| E08A0209-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Annexin V ELISA kit |
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| E08A0593-192T | BlueGene | 192 tests | 1524 EUR |
|
Description: A competitive ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Annexin V ELISA kit |
|||
| E08A0593-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A competitive ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Annexin V ELISA kit |
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| E08A0593-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A competitive ELISA for quantitative measurement of Canine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Annexin V ELISA kit |
|||
| E07A0593-192T | BlueGene | 192 tests | 1524 EUR |
|
Description: A competitive ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Annexin V ELISA kit |
|||
| E07A0593-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A competitive ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Annexin V ELISA kit |
|||
| E07A0593-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A competitive ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Annexin V ELISA kit |
|||
| E07A0209-192T | BlueGene | 192 tests | 1524 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Annexin V ELISA kit |
|||
| E07A0209-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Annexin V ELISA kit |
|||
| E07A0209-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rat Annexin V ELISA kit |
|||
| E02A0593-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Annexin V ELISA kit |
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| E02A0593-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Annexin V ELISA kit |
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| E02A0593-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Annexin V ELISA kit |
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| E02A0209-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A sandwich ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Annexin V ELISA kit |
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| E02A0209-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Annexin V ELISA kit |
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| E02A0209-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A sandwich ELISA for quantitative measurement of Rat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Annexin V ELISA kit |
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| E06A0209-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A sandwich ELISA for quantitative measurement of Goat Annexin V in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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The results of intraplantar and intrathecal botulinum toxin sort B on tactile allodynia in mono and polyneuropathy in the mouse.
BACKGROUND
Mononeuropathies (MNs: nerve ligation) and polyneuropathies (PNs: cisplatin) produce unilateral and bilateral tactile allodynia, respectively. We examined the consequences of intraplantar (IPLT) and intrathecal (IT) botulinum toxin B (BoNT-B) on this allodynia.
METHODS
Mice (male c57Bl/6) have been ready with an L5 nerve ligation. Others obtained cisplatin (IP 2.Three mg/kg/d, each different day for six injections). Saline and BoNT-B have been administered via the IPLT or IT route. We examined mechanical allodynia (von Frey hairs) earlier than and at intervals after BoNT. As a management, we injected IPLT BoNT-B handled with dithiothreitol to cleave heavy chain from gentle chain. We measured motor operate utilizing acute thermal escape and sensorimotor assessments.
RESULTS
MN and PN mice confirmed a persistent ipsilateral and bilateral allodynia, respectively. IPLT BoNT-B resulted in an ipsilateral dorsal horn discount in the synaptic protein goal of BoNT-B (vesicle-associated membrane protein) and a long-lasting (as much as roughly 17 days) reversal of allodynia in PN and MN fashions. The predominant impact after IPLT supply was ipsilateral to IPLT BoNT. The results of IPLT BoNT-B in MN mice have been blocked by prior discount of BoNT-B with dithiothreitol.
IT BoNT-B in mice with PN resulted in a bilateral reversal of allodynia. With these dosing parameters, hind paw putting and stepping reflexes have been unaltered, and there have been no modifications in thermal escape latencies. After cisplatin, dorsal root ganglions displayed will increase in activation transcription issue 3, which have been diminished by IT, however not IPLT BoNT-B.
CONCLUSIONS
BoNT-B given IPLT and IT yields a long-lasting attenuation of the allodynia in mice displaying MN and PN allodynia.